Kishi H, Okada T, Otsuka M, Watanabe G, Taya K, Sasamoto S
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Japan.
J Endocrinol. 1996 Oct;151(1):65-75. doi: 10.1677/joe.0.1510065.
The present study was conducted to study the effect of immunoneutralization against endogenous inhibin on FSH, LH, oestradiol-17 beta and progesterone secretion and to investigate the effect of removal of endogenous inhibin on subsequent follicular development in the hamster. After treatment with anti-inhibin serum (inhibin-AS) at 1100 h on day 2 of the oestrous cycle (day 1 = day of ovulation), a marked increase in plasma levels of FSH and a slight increase in plasma levels of LH were noted and pituitary contents of FSH, but not LH, were also increased. In the group treated with inhibin-AS, superovulation occurred on day 1 of the following cycle. Plasma levels of oestradiol-17 beta markedly increased with the increase in the number of ovulations induced by human chorionic gonadotrophin (hCG) as compared with those in control animals. In the second cycle, plasma concentrations and pituitary contents of FSH in the animals given 200 microliters inhibin-AS still showed high values as compared with those in the animals treated with control serum, although superovulation did not occur on day 1 of the third cycle. Plasma concentrations and pituitary contents of LH in the hamster given 200 microliters inhibin-AS tended to decrease as compared with those in control animals during the second cycle. Plasma concentrations of oestradiol-17 beta in the animals treated with 200 microliters inhibin-AS changed in a similar way to controls. A marked increase in plasma concentrations of progesterone was noted on days 1 and 2 of the second cycle in the group receiving inhibin-AS. The twice daily injection of 1 IU hCG during the second cycle to the animals given 200 microliters inhibin-AS induced superovulation on day 1 of the third cycle. These results indicate that circulating inhibin may be an important indicator of the number of developing follicles and may maintain the species-specific number of developing follicles through suppression of FSH secretion in the cyclic hamster. They also suggest that high levels of inhibin slightly suppress plasma levels of LH, indicating that plasma LH may also regulate follicular development in the cyclic hamster.
本研究旨在探讨对内源性抑制素进行免疫中和对促卵泡素（FSH）、促黄体素（LH）、雌二醇-17β和孕酮分泌的影响，并研究去除内源性抑制素对仓鼠后续卵泡发育的影响。在发情周期第2天（第1天为排卵日）的1100时用抗抑制素血清（抑制素-AS）处理后，观察到血浆FSH水平显著升高，血浆LH水平略有升高，垂体中FSH含量增加，但LH含量未增加。在接受抑制素-AS处理的组中，在下一个周期的第1天出现了超排卵。与对照动物相比，随着人绒毛膜促性腺激素（hCG）诱导的排卵数增加，血浆雌二醇-17β水平显著升高。在第二个周期中，给予200微升抑制素-AS的动物的血浆FSH浓度和垂体FSH含量与给予对照血清的动物相比仍显示出较高的值，尽管在第三个周期的第1天未出现超排卵。在第二个周期中，给予200微升抑制素-AS的仓鼠的血浆LH浓度和垂体LH含量与对照动物相比有下降趋势。给予200微升抑制素-AS的动物的血浆雌二醇-17β浓度变化与对照动物相似。在接受抑制素-AS的组中，在第二个周期的第1天和第2天观察到血浆孕酮浓度显著升高。在第二个周期中，每天两次给给予200微升抑制素-AS的动物注射1国际单位hCG，在第三个周期的第1天诱导了超排卵。这些结果表明，循环中的抑制素可能是发育卵泡数量的重要指标，并可能通过抑制周期性仓鼠的FSH分泌来维持特定物种发育卵泡的数量。它们还表明，高水平的抑制素会轻微抑制血浆LH水平，表明血浆LH也可能调节周期性仓鼠的卵泡发育。
