Gahl W A, Kozina T J, Fuhrmann D D, Vale A M
Obstet Gynecol. 1982 Sep;60(3):297-304.
Diamine oxidase activity is present in amniotic fluid but absent from vaginal secretions, providing a method for diagnosing rupture of membranes. Vaginal secretions were absorbed onto sterile paper strips, eluted, and assayed for diamine oxidase activity. Interfering substances included maternal serum, iodine antiseptics, and large amounts of meconium. Two of 109 prenatal clinic (negative control) specimens displayed measurable diamine oxidase activity; one of 144 postamniotomy (positive control) specimens had no diamine oxidase activity. Of 75 test specimens obtained by speculum examination and 488 test specimens obtained without the aid of a speculum, 18 and 62, respectively, displayed a diamine oxidase test result different from results of conventional tests (nitrazine paper, ferning, fetal cell stains). These discrepancies are discussed in view of the utility of the diamine oxidase test as an adjunct to conventional methods for diagnosing rupture of membranes. The diamine oxidase test appears to be objective, sensitive, quantitative, and capable of detecting amniotic fluid leaks more than 25 hours after rupture of membranes. It can be performed in 1 hour and requires a scintillation counter.
二胺氧化酶活性存在于羊水之中，但阴道分泌物中不存在，这为诊断胎膜破裂提供了一种方法。将阴道分泌物吸附到无菌纸条上，洗脱后检测二胺氧化酶活性。干扰物质包括母体血清、碘消毒剂和大量胎粪。109份产前诊所（阴性对照）标本中有2份显示出可测量的二胺氧化酶活性；144份羊膜穿刺术后（阳性对照）标本中有1份没有二胺氧化酶活性。在通过窥器检查获得的75份检测标本和未借助窥器获得的488份检测标本中，分别有18份和62份显示二胺氧化酶检测结果与传统检测方法（硝嗪纸、羊齿状结晶、胎儿细胞染色）的结果不同。鉴于二胺氧化酶检测作为诊断胎膜破裂的传统方法的辅助手段的实用性，对这些差异进行了讨论。二胺氧化酶检测似乎是客观、灵敏、定量的，并且能够检测到胎膜破裂25小时以上后的羊水渗漏。它可以在1小时内完成，并且需要一台闪烁计数器。
