Clos J, Legrand C, Legrand J, Ghandour M S, Labourdette G, Vincendon G, Gombos G
Dev Neurosci. 1982 Mar-Jun;5(2-3):285-92. doi: 10.1159/000112687.
The developmental pattern of S100 protein, a specific marker of astroglia, was studied by radioimmunoassay in the cerebellum of thyroid-deficient, thyroxine-treated and undernourished rats during development. In the control animals, the S100 protein content of the cerebellum increased maximally after the 3rd postnatal week, i.e., after cell multiplication had stopped and when the cerebellum had acquired more than 70% of its adult weight and protein content. This developmental pattern of S100 protein reflected essentially the maturation of astroglia. In the thyroxine-treated rats the total amount and the concentration of S100 protein were higher than in controls during the first 3 weeks of postnatal life and returned to normal values thereafter. In the thyroid-deficient rats both the amount and concentration were lower than in controls throughout development. In the undernourished animals the amount of S100 protein per organ was also lower than in controls during the whole experimental period studied; the S100 protein concentration was higher during the first 2 postnatal weeks and became lower thereafter. These results are discussed taking into consideration previous histological and ultrastructural observations on the effects of altered thyroid state and undernutrition on the formation and maturation of cerebellar astrocytes.
采用放射免疫分析法，研究了甲状腺功能减退、经甲状腺素治疗和营养不良的大鼠在发育过程中，小脑内星形胶质细胞特异性标志物S100蛋白的发育模式。在对照动物中，小脑的S100蛋白含量在出生后第3周后达到最大值，即在细胞增殖停止后，且小脑已达到其成年体重和蛋白质含量的70%以上时。S100蛋白的这种发育模式基本上反映了星形胶质细胞的成熟。在经甲状腺素治疗的大鼠中，出生后前3周S100蛋白的总量和浓度高于对照组，此后恢复到正常水平。在甲状腺功能减退的大鼠中，整个发育过程中S100蛋白的量和浓度均低于对照组。在营养不良的动物中，在所研究的整个实验期间，每个器官的S100蛋白量也低于对照组；出生后前2周S100蛋白浓度较高，此后降低。结合先前关于甲状腺状态改变和营养不良对小脑星形胶质细胞形成和成熟影响的组织学和超微结构观察结果，对这些结果进行了讨论。
