Ghandour M S, Labourdette G, Vincendon G, Gombos G
Dev Neurosci. 1981;4(2):98-109. doi: 10.1159/000112745.
Specific immune sera, one directed against S100 protein and one against glial fibrillary acidic protein (both proteins are specific astrocytic markers in rat cerebellum), were used in immunohistological methods to follow the formation and maturation of astrocytes during the postnatal development of rat cerebellum. Throughout development, S100 protein is detected only in astrocytes. Immunofluorescent Golgi epithelial cells and other astrocytes are detected in the cerebellum of newborn rat, their number increases rapidly between birth and the 10th postnatal day and then more slowly until the end of the 3rd week. In contrast, the increase in S100 protein level, measured in cerebellar homogenates by radioimmunoassay, was small until the 10th postnatal day. The greater accumulation subsequently found corresponds to an increase in the content of each astrocyte during the process of maturation.
使用了两种特异性免疫血清，一种针对S100蛋白，另一种针对胶质纤维酸性蛋白（这两种蛋白都是大鼠小脑星形胶质细胞的特异性标志物），通过免疫组织学方法追踪大鼠小脑出生后发育过程中星形胶质细胞的形成和成熟。在整个发育过程中，仅在星形胶质细胞中检测到S100蛋白。在新生大鼠的小脑中检测到免疫荧光的高尔基上皮细胞和其他星形胶质细胞，其数量在出生至出生后第10天之间迅速增加，然后增加速度减慢，直至第3周结束。相比之下，通过放射免疫测定法在小脑匀浆中测量的S100蛋白水平在出生后第10天之前升高幅度较小。随后发现的更大积累量对应于成熟过程中每个星形胶质细胞含量的增加。
