一种分离视网膜S抗原的简单快速方法。
A simple and rapid method for isolation of retinal S antigen.
作者信息
Dorey C, Cozette J, Faure J P
出版信息
Ophthalmic Res. 1982;14(4):249-55. doi: 10.1159/000265199.
PMID:6813787
Abstract
A procedure is described for the rapid isolation of S antigen from retinal tissue, by salt precipitation, gel filtration and final purification by adsorption chromatography on hydroxyapatite-agarose. The method yielded a highly purified material and allowed an excellent preservation of the antigenicity, as shown by high pathogenic activity in induction of experimental autoimmune uveoretinitis.
摘要
本文描述了一种从视网膜组织中快速分离S抗原的方法，该方法包括盐沉淀、凝胶过滤以及最终通过羟基磷灰石-琼脂糖吸附色谱法进行纯化。该方法得到了高度纯化的物质，并能很好地保留抗原性，这在诱导实验性自身免疫性葡萄膜视网膜炎中表现出高致病活性。
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