Ikawa M, Wegener K, Foxall T L, Sasner J J
Toxicon. 1982;20(4):747-52. doi: 10.1016/0041-0101(82)90122-2.
A toxic strain of Aphanizomenon flos-aquae (NH-1), isolated from a toxic bloom in a pond in Durham, New Hampshire, has been mass cultured in the laboratory. The toxin was extracted by ultrasonic disruption of the cells and purified by; (a) filtration through a 10 kilodalton filter, and (b) chromatography on a strong cation exchange resin column using 0.01 M, then 0.1 M, pH 5, sodium acetate buffer followed by 0.75 M hydrochloric acid. Mouse assays and fluorescence generated by hydrogen peroxide oxidation were used to monitor the fractions. Only a nonfluorescent toxic peak followed immediately by a fluorescent less-toxic peak were detected, both eluting with the hydrochloric acid fractions. The toxins were identical in behavior to neosaxitoxin and saxitoxin, respectively, when compared with elution profiles of the paralytic shellfish poisons from Gonyaulax tamarensis var. excavata and by paper electrophoretic and thin-layer chromatographic comparisons. The toxin profile appears to be different from that of a previously isolated strain of A. flos-aquae from Kezar Lake.
从新罕布什尔州达勒姆一个池塘的有毒水华中分离出的水华鱼腥藻有毒菌株（NH-1）已在实验室中进行大规模培养。通过超声破碎细胞提取毒素，并通过以下方法进行纯化：（a）通过10千道尔顿滤膜过滤，以及（b）在强阳离子交换树脂柱上进行色谱分离，使用0.01 M，然后0.1 M，pH 5的醋酸钠缓冲液，接着使用0.75 M盐酸。使用小鼠试验和过氧化氢氧化产生的荧光来监测各馏分。仅检测到一个非荧光毒性峰，紧接着是一个荧光较弱的毒性峰，两者均随盐酸馏分洗脱。与塔玛亚历山大藻变种（Gonyaulax tamarensis var. excavata）麻痹性贝类毒素的洗脱图谱相比，以及通过纸电泳和薄层色谱比较，这些毒素的行为分别与新石房蛤毒素和石房蛤毒素相同。毒素谱似乎与之前从凯扎尔湖分离出的水华鱼腥藻菌株不同。
