Mahmood N A, Carmichael W W
Toxicon. 1986;24(2):175-86. doi: 10.1016/0041-0101(86)90120-0.
A single filament clonal isolate of Aphanizomenon flos-aquae was made from a water bloom sample taken at a small pond near Durham, New Hampshire, in 1980. When batch cultured the strain was toxic to mice and had an i.p. LD50 of about 5.0 mg/kg. Using an extraction procedure originally designed for paralytic shellfish poisons and other neurotoxins of freshwater cyanobacteria, a purification method was developed. The procedure involved acidified water/ethanol extraction of the cells followed by ultrafiltration, gel filtration, use of C18 cartridges to remove pigments, ion-exchange and high performance liquid chromatography using u.v. detection at 220 or 240 nm. Thin-layer chromatography and high performance liquid chromatography results indicate that Aphanizomenon flos-aquae NH-5 may produce paralytic shellfish poisons, mainly neo-saxitoxin and saxitoxin. Three labile toxins were also detected which were not similar to any of the known paralytic shellfish poisons.
1980年，从新罕布什尔州达勒姆附近一个小池塘采集的水华样本中分离出一株水华束丝藻单丝克隆菌株。该菌株在分批培养时对小鼠有毒，腹腔注射半数致死剂量（LD50）约为5.0毫克/千克。利用最初为麻痹性贝类毒素和淡水蓝藻的其他神经毒素设计的提取程序，开发了一种纯化方法。该程序包括用酸化水/乙醇提取细胞，然后进行超滤、凝胶过滤、使用C18柱去除色素、离子交换以及在220或240纳米处进行紫外检测的高效液相色谱。薄层色谱和高效液相色谱结果表明，水华束丝藻NH - 5可能产生麻痹性贝类毒素，主要是新石房蛤毒素和石房蛤毒素。还检测到三种不稳定毒素，它们与任何已知的麻痹性贝类毒素都不相似。
