Thibault P, Pleasance S, Laycock M V
Institute for Murine Biosciences, National Research Council of Canada, Halifax, Nova Scotia.
J Chromatogr. 1991 Apr 12;542(2):483-501. doi: 10.1016/s0021-9673(01)88783-5.
A capillary electrophoresis (CE) method with UV detection is described for the separation and determination of underivatized toxins associated with paralytic shellfish poisoning (PSP). Confirmation of the electrophoretic peaks was facilitated by mass spectrometric (MS) detection using an ionspray CE-MS interface and by high-performance liquid chromatography with fluorescence detection. The determination of PSP toxins, such as saxitoxin and neosaxitoxin, in toxic dinoflagellates and scallops is demonstrated and comparisons are made with existing techniques.
本文描述了一种采用紫外检测的毛细管电泳（CE）方法，用于分离和测定与麻痹性贝类中毒（PSP）相关的未衍生化毒素。通过使用离子喷雾CE-MS接口的质谱（MS）检测以及带有荧光检测的高效液相色谱法，有助于对电泳峰进行确认。文中展示了该方法用于测定有毒甲藻和扇贝中PSP毒素（如石房蛤毒素和新石房蛤毒素）的情况，并与现有技术进行了比较。
