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大鼠中枢神经系统中的白三烯

Leukotrienes in the rat central nervous system.

作者信息

Lindgren J A, Hökfelt T, Dahlén S E, Patrono C, Samuelsson B

出版信息

Proc Natl Acad Sci U S A. 1984 Oct;81(19):6212-6. doi: 10.1073/pnas.81.19.6212.

DOI:10.1073/pnas.81.19.6212
PMID:6091141
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC391890/
Abstract

Leukotrienes C4, D4, and E4 were isolated after incubation of rat brain tissue in vitro with the ionophore A23187 and arachidonic acid. Identification of the compounds was carried out using high-performance liquid chromatography, radioimmunoassay, and bioassay. Average production of leukotrienes C4, D4, and E4 during 10 min of incubation was estimated to 25, 8, and 0.7 pmol per g of brain tissue (wet weight), respectively. Radioimmunoassay determinations indicated in vitro biosynthesis of leukotriene C4 in most regions of the brain, with the highest levels obtained in the hypothalamus and the median eminence. In slices from the caudate nucleus, ionophore A23187 caused a dose-dependent stimulation of leukotriene C4 formation with maximal effect at 5 microM. Leukotriene C4 synthesis of rat brain tissue was inhibited by 30 microM nordihydroguaiaretic acid. Finally, using the indirect immunofluorescence technique, nerve endings in the median eminence and cell bodies in the preoptic area reacting with antibodies raised against leukotriene C4 were observed.

摘要

在体外将大鼠脑组织与离子载体A23187和花生四烯酸一起孵育后，分离出白三烯C4、D4和E4。使用高效液相色谱、放射免疫测定法和生物测定法对这些化合物进行鉴定。在孵育10分钟期间，白三烯C4、D4和E4的平均生成量估计分别为每克脑组织（湿重）25、8和0.7皮摩尔。放射免疫测定结果表明，脑的大多数区域均可在体外生物合成白三烯C4，其中下丘脑和正中隆起中的水平最高。在尾状核切片中，离子载体A23187可引起白三烯C4生成的剂量依赖性刺激，在5微摩尔时效果最佳。30微摩尔的去甲二氢愈创木酸可抑制大鼠脑组织白三烯C4的合成。最后，使用间接免疫荧光技术，观察到正中隆起处的神经末梢和视前区中与抗白三烯C4抗体发生反应的细胞体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/661897c35019/pnas00620-0311-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/cf3b32811d1e/pnas00620-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/e094f5224ef8/pnas00620-0311-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/5fa5a8155545/pnas00620-0311-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/588e5d9dc40c/pnas00620-0311-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/3b8b6dcfe949/pnas00620-0311-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/661897c35019/pnas00620-0311-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/cf3b32811d1e/pnas00620-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/e094f5224ef8/pnas00620-0311-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/5fa5a8155545/pnas00620-0311-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/588e5d9dc40c/pnas00620-0311-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/3b8b6dcfe949/pnas00620-0311-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0492/391890/661897c35019/pnas00620-0311-f.jpg

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本文引用的文献

1
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Biochim Biophys Acta. 1964 Apr 20;84:218-9.
2
Fluorescent antibody methods.荧光抗体法
Gen Cytochem Methods. 1958;1:399-422.
3
Effect of temperature and antioxidants upon the lipoxidase-catalyzed oxidation of sodium linoleate.温度和抗氧化剂对脂肪氧合酶催化亚油酸钠氧化的影响。
小鼠小脑浦肯野细胞中的 5-脂氧合酶。
Neuroscience. 2010 Dec 1;171(2):383-9. doi: 10.1016/j.neuroscience.2010.09.019. Epub 2010 Sep 17.
4
Advances in analytical mass spectrometry to improve screening for inherited metabolic diseases.用于改善遗传性代谢疾病筛查的分析质谱技术进展。
Eur J Pediatr. 2003 Dec;162 Suppl 1:S67-76. doi: 10.1007/s00431-003-1356-y. Epub 2003 Nov 14.
5
Production of leukotrienes in a model of focal cerebral ischaemia in the rat.大鼠局灶性脑缺血模型中白三烯的产生
Br J Pharmacol. 2001 Aug;133(8):1323-9. doi: 10.1038/sj.bjp.0704189.
6
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J Inherit Metab Dis. 2000 Jun;23(4):404-8. doi: 10.1023/a:1005664204956.
7
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Brain Pathol. 2000 Jan;10(1):113-26. doi: 10.1111/j.1750-3639.2000.tb00248.x.
8
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9
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4
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6
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8
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10
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