Hill R J, Mott M R, Burnett E J, Abmayr S M, Lowenhaupt K, Elgin S C
J Cell Biol. 1982 Oct;95(1):262-6. doi: 10.1083/jcb.95.1.262.
The regularly repeating periodic nucleosome organization is clearly resolved in the chromatin of the isolated salivary chromosomes of Drosophila melanogaster. A new microsurgical procedure of isolation in buffer A of Hewish and Burgoyne (1973, Biochem. Biophys. Res. Commun., 52:504-510) yielded native Drosophila salivary chromosomes. These chromosomes were then swollen and spread by a modified Miller procedure, stained or shadowed, and examined in the electron microscope. Individual nucleoprotein fibers were resolved with regularly repeated nucleosomes of approximately 10 nm diameter. Micrococcal nuclease digestion of isolated salivary nuclei gave a family of DNA fragments characteristic of nucleosomes for total chromatin, 5S gene, and simple satellite (rho = 1.688 g/cm3) sequences.
在黑腹果蝇分离的唾液腺染色体的染色质中，规则重复的周期性核小体组织清晰可见。采用休伊什和伯戈因（1973年，《生物化学与生物物理研究通讯》，52:504 - 510）的缓冲液A中的一种新的显微外科分离程序，得到了天然的果蝇唾液腺染色体。然后通过改良的米勒程序使这些染色体膨胀并展开，进行染色或投影，并用电子显微镜检查。单个核蛋白纤维可分辨出直径约为10纳米的规则重复的核小体。对分离的唾液腺细胞核进行微球菌核酸酶消化，得到了一组对于总染色质、5S基因和简单卫星（ρ = 1.688 g/cm³）序列而言具有核小体特征的DNA片段。
