通过立体电子显微镜观察薄切片中的核小体。
Visualization of nucleosomes in thin sections by stereo electron microscopy.
作者信息
Olins A L, Olins D E, Zentgraf H, Franke W W
出版信息
J Cell Biol. 1980 Dec;87(3 Pt 1):833-6. doi: 10.1083/jcb.87.3.833.
Abstract
Nucleosomes (approximately diameter) were clearly visualized in thin sections (approximately 0.1 micrometer thick) of isolated chicken erythrocytes. The cells were lysed and fixed in low ionic strength buffers that maintained the chromatin as dispersed filaments and prevented the reformation of supranucleosomal structures. Stereo electron micrographs at high magnification demonstrate the stability of nucleosome structure in the dispersed chromatin state during fixation, dehydration, and embedding.
摘要
在分离出的鸡红细胞的薄切片（约0.1微米厚）中可以清晰地看到核小体（直径约）。细胞被裂解并固定在低离子强度缓冲液中，该缓冲液可使染色质保持为分散的细丝状，并防止超核小体结构的重新形成。高倍立体电子显微镜照片显示了在固定、脱水和包埋过程中，处于分散染色质状态的核小体结构的稳定性。
相似文献
1
Visualization of nucleosomes in thin sections by stereo electron microscopy.通过立体电子显微镜观察薄切片中的核小体。
J Cell Biol. 1980 Dec;87(3 Pt 1):833-6. doi: 10.1083/jcb.87.3.833.
2
Chromatin organization in detergent-lysed chicken erythrocyte nuclei.去污剂裂解的鸡红细胞核中的染色质组织
J Electron Microsc Tech. 1987 Nov;7(3):223-31. doi: 10.1002/jemt.1060070311.
3
4
A repeating unit of higher order chromatin structure in chick red blood cell nuclei.鸡红细胞核中高阶染色质结构的重复单元。
Chromosoma. 1980;78(3):257-74. doi: 10.1007/BF00327387.
5
Differences of supranucleosomal organization in different kinds of chromatin: cell type-specific globular subunits containing different numbers of nucleosomes.不同类型染色质中超核小体组织的差异：含有不同数量核小体的细胞类型特异性球状亚基。
J Cell Biol. 1984 Jul;99(1 Pt 1):272-86. doi: 10.1083/jcb.99.1.272.
6
Supranucleosomal fiber loops of chicken erythrocyte chromatin.鸡红细胞染色质的超核小体纤维环
J Electron Microsc (Tokyo). 1984;33(2):178-81.
7
Supranucleosomal organization of chromatin. Electron microscopic visualization of long polynucleosomal chains.染色质的超核小体组织。长多聚核小体链的电子显微镜观察。
Chromosoma. 1982;85(2):251-60. doi: 10.1007/BF00294969.
8
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.组蛋白H1参与核小体的组装以及染色质盐依赖性超结构的形成。
J Cell Biol. 1979 Nov;83(2 Pt 1):403-27. doi: 10.1083/jcb.83.2.403.
9
The structure of the nucleosome core particle of chromatin in chicken erythrocytes visualized by using atomic force microscopy.利用原子力显微镜观察鸡红细胞染色质核小体核心颗粒的结构。
Cell Res. 1999 Dec;9(4):255-60. doi: 10.1038/sj.cr.7290024.
10
Stereo electron microscopy of the 25-nm chromatin fibers in isolated nuclei.分离细胞核中25纳米染色质纤维的立体电子显微镜观察。
J Cell Biol. 1979 Apr;81(1):260-5. doi: 10.1083/jcb.81.1.260.
引用本文的文献
1
Spatial localization of genes determined by intranuclear DNA fragmentation with the fusion proteins lamin KRED and histone KRED und visible light.用融合蛋白 lamin KRED 和 histone KRED 及可见光使核内 DNA 碎片化来确定基因的空间定位。
Int J Med Sci. 2013 Jul 7;10(9):1136-48. doi: 10.7150/ijms.6121. Print 2013.
2
The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography.通过电子显微镜断层扫描技术观察体外重构的非洲爪蟾染色体的三维结构。
Chromosoma. 2007 Aug;116(4):349-72. doi: 10.1007/s00412-007-0101-0. Epub 2007 Feb 28.
3
Nucleosome repeat structure is present in native salivary chromosomes of Drosophila melanogaster.核小体重复结构存在于黑腹果蝇的天然唾液腺染色体中。
J Cell Biol. 1982 Oct;95(1):262-6. doi: 10.1083/jcb.95.1.262.
4
The layered organization of nucleosomes in 30 nm chromatin fibers.30纳米染色质纤维中核小体的分层组织。
Chromosoma. 1985;91(5):377-90. doi: 10.1007/BF00291012.
本文引用的文献
1
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
2
Effects of low salt concentration on structural organization and template activity of chromatin in chicken erythrocyte nuclei.低盐浓度对鸡红细胞核染色质结构组织和模板活性的影响。
Exp Cell Res. 1971 Mar;65(1):61-72. doi: 10.1016/s0014-4827(71)80050-2.
3
The relation between the unit thread of chromosomes and isolated nucleohistone.染色体的单位细丝与分离的核组蛋白之间的关系。
J Mol Biol. 1974 Jan 5;85(4):533-49. doi: 10.1016/0022-2836(74)90314-3.
4
Spheroid chromatin units (v bodies).球状染色质单位（V小体）。
Science. 1974 Jan 25;183(4122):330-2. doi: 10.1126/science.183.4122.330.
5
Involvement of vesicle coat material in casein secretion and surface regeneration.囊泡被膜物质在酪蛋白分泌和表面再生中的作用。
J Cell Biol. 1976 Apr;69(1):173-95. doi: 10.1083/jcb.69.1.173.
6
Chromatin nu bodies: isolation, subfractionation and physical characterization.染色质核小体：分离、亚分级分离及物理特性分析
Nucleic Acids Res. 1976 Dec;3(12):3271-91. doi: 10.1093/nar/3.12.3271.
7
Levels of granular organization of chromatin fibres.染色质纤维的颗粒组织水平。
FEBS Lett. 1976 Sep 1;67(3):323-7. doi: 10.1016/0014-5793(76)80557-1.
8
Structural repeating units in chromatin. I. Evidence for their general occurrence.染色质中的结构重复单元。I. 其普遍存在的证据。
Exp Cell Res. 1976 Jan;97:101-10. doi: 10.1016/0014-4827(76)90659-5.
9
Nucleosome arcs and helices.核小体弧和螺旋。
Science. 1978 Oct 20;202(4365):280-6. doi: 10.1126/science.694532.
10
Stereo-electron microscopy and energy-dispersive x-ray analysis of avian reticulocytes.禽类网织红细胞的立体电子显微镜检查及能量色散X射线分析
Eur J Cell Biol. 1979 Aug;19(3):239-49.
Zotero 插件