Kubagawa H, Gathings W E, Levitt D, Kearney J F, Cooper M D
J Clin Immunol. 1982 Oct;2(4):264-9. doi: 10.1007/BF00915065.
We have undertaken an immunofluorescent analysis of the immunoglobulin heavy and light chains expressed by pre-B cells from normal human fetal and adult bone marrow using purified mouse monoclonal and goat antibodies to human immunoglobulin isotypes. Our results indicate that (i) the great majority of normal pre-B cells in both fetuses and adults expresses intracytoplasmic mu chains only, (ii) immunoglobulin light-chain synthesis may be initiated during a late stage in pre-B-cell development, and (iii) heavy-chain isotype switching at the pre-B-cell stage may not occur during normal B-cell development.
我们使用纯化的小鼠单克隆抗体和山羊抗人免疫球蛋白同种型抗体，对来自正常人类胎儿和成人骨髓的前B细胞所表达的免疫球蛋白重链和轻链进行了免疫荧光分析。我们的结果表明：（i）胎儿和成人中绝大多数正常前B细胞仅表达胞质内的μ链；（ii）免疫球蛋白轻链的合成可能在前B细胞发育的晚期开始；（iii）在前B细胞阶段，正常B细胞发育过程中可能不会发生重链同种型转换。
