Schössler W, Stepanauskas M, Dittrich C, von Chr Eichhorn M
Acta Biol Med Ger. 1982;41(7-8):695-703.
We describe an enzyme-immunoassay for the determination of factor VIII antigen. After representation of the isolation of proteins the enzyme-immunoassay is presented. The principle of the method is the following: Test plasma is mixed with rabbit antibody in excess and incubated at 37 degrees C. The incubation mixture is added to polystyrene tubes, which are coated with human factor VIII. The rabbit antibody is available to adhere to factor VIII coating the tube and can be detected with an enzyme-labeled antibody to rabbit IgG. This method is sensitive to 7.8 . 10(-3) U/ml factor VIII antigen; the variation coefficient is 10.9%.
我们描述了一种用于测定凝血因子 VIII 抗原的酶免疫测定法。在介绍蛋白质分离方法后，展示了酶免疫测定法。该方法的原理如下：将测试血浆与过量的兔抗体混合，并在 37℃下孵育。将孵育混合物加入到包被有人凝血因子 VIII 的聚苯乙烯管中。兔抗体可与包被在管上的凝血因子 VIII 结合，并可用酶标记的抗兔 IgG 抗体进行检测。该方法对凝血因子 VIII 抗原的检测灵敏度为 7.8×10⁻³U/ml；变异系数为 10.9%。
