Ness P M, Perkins H A
Thromb Haemost. 1979 Oct 31;42(3):848-54.
An enzyme immunoassay (EIA) system has been developed to measure factor VIII-related antigen (VIIIAGN). This assay gives similar results to the commonly used Laurell electroimmunodiffusion (EID) assay for VIIIAGN as shown by comparison of both techniques with samples from healthy controls, patients with hemophilia A, and patients with von Willebrand's disease. The assay also has a greater precision than the EID technique as demonstrated by multiple assays of aliquots of a single sample. The use of this EIA test for VIIIAGN is simple and employs inexpensive reagents and equipment. The use of expensive antisera is minimized. EIA for VIIIAGN has the advantage of increased sensitivity compared to Laurell EIA.
已开发出一种酶免疫测定（EIA）系统来检测因子VIII相关抗原（VIIIAGN）。通过将该检测方法与来自健康对照、甲型血友病患者和血管性血友病患者的样本进行比较，结果表明，该检测方法对于VIIIAGN所给出的结果与常用的 Laurell 电免疫扩散（EID）检测方法相似。如对单个样本的等分试样进行多次检测所示，该检测方法也比EID技术具有更高的精密度。用于检测VIIIAGN的这种EIA检测方法操作简单，使用的试剂和设备价格低廉。昂贵抗血清的使用降至最低。与 Laurell EIA相比，用于VIIIAGN的EIA具有灵敏度更高的优势。
