Micheel B, Karsten U, Fiebach H
Acta Histochem. 1982;71(1):15-8. doi: 10.1016/S0065-1281(82)80005-6.
A solid-phase immunofluorescence assay (SIFA) is presented which allows the demonstration of antibodies against soluble antigens. The test is performed by successively incubating nitrate cellulose discs in microtitration plates with anti-Ig, the sample to be tested and FITC-labeled antigen. The fluorescence intensity of the discs is then measured by using a microfluorometer. The assay is simple, needs a small amount of material and allows to test a large number of samples.
本文介绍了一种固相免疫荧光测定法（SIFA），该方法可用于检测针对可溶性抗原的抗体。检测过程是在微量滴定板中依次将硝酸纤维素圆盘与抗Ig、待测样品以及异硫氰酸荧光素（FITC）标记的抗原进行孵育。然后使用微量荧光计测量圆盘的荧光强度。该测定法操作简单，所需材料量少，且能够检测大量样品。
