Amorena B, Stone W H
Anim Blood Groups Biochem Genet. 1982;13(2):81-90. doi: 10.1111/j.1365-2052.1982.tb01046.x.
Sera from about 1000 cows were tested for cytotoxicity against a panel of up to 100 lymphocyte samples. Cytotoxic antibodies presumably resulting from transplacental immunization of the cow by her calf were found in about 45% of these sera. The antibody titers of sera from parous cows rarely exceed 4(2), some persisted for over one year, but decreased notably at calving. Thirty-five immune sera were also produced by alloimmunization with lymphocytes. They usually reached peak titers of up to 4(4) at 2 or 3 weeks after the initial immunization. Subsequent immunizations produced sera with very high titers but they were much more polyspecific. High-titered antibodies were also produced by skin graft recipients. Useful cytotoxic antibodies were found in 19 of 111 colostrum whey samples. Studies on 13 dam-calf pairs showed that the newborn calf may acquire cytotoxic antibodies from its mother's colostrum, but the only cytotoxic antibodies detectable in this calf's serum are those not directed against its own lymphocyte antigens. It is concluded that efficient lymphocyte typing requires antibodies from a variety of sources.
检测了约1000头奶牛的血清对多达100个淋巴细胞样本的细胞毒性。在这些血清中，约45%发现了可能由犊牛经胎盘免疫母牛产生的细胞毒性抗体。经产母牛血清的抗体效价很少超过4（2），有些持续一年以上，但在产犊时显著下降。还用淋巴细胞同种免疫产生了35份免疫血清。它们通常在初次免疫后2或3周达到高达4（4）的峰值效价。随后的免疫产生的血清效价非常高，但多特异性更强。皮肤移植受者也产生了高效价抗体。在111份初乳乳清样本中，有19份发现了有用的细胞毒性抗体。对13对母犊牛的研究表明，新生犊牛可能从其母亲的初乳中获得细胞毒性抗体，但在该犊牛血清中可检测到的唯一细胞毒性抗体是那些不针对其自身淋巴细胞抗原的抗体。结论是，有效的淋巴细胞分型需要来自多种来源的抗体。
