Hartmann C, Rode A
C R Seances Acad Sci III. 1982 Sep 20;295(2):69-72.
The determination of the buoyant density of synchronously dividing Chick embryo fibroblast DNA, substituted at various times during the cell cycle by 5-bromodeoxyuridine (BrdU), has been carried out by means of analytical neutral CsCl banding. It has been shown that the buoyant density of BrdU-substituted DNA, duplicated during the first half of S phase, was 0.010 g/ml higher than that of BrdU-substituted DNA duplicated during the second half of S phase. That may reflect a preferential incorporation of the analogue at the beginning of S phase. This peculiar pattern of incorporation would be responsible for the observed inhibition of cell transformation occurring when fibroblasts, infected by Rous sarcoma virus, are grown in the presence of the analogue.
通过分析性中性氯化铯密度梯度离心法，对在细胞周期不同时间被5-溴脱氧尿苷（BrdU）取代的同步分裂鸡胚成纤维细胞DNA的浮力密度进行了测定。结果表明，在S期前半段复制的BrdU取代DNA的浮力密度比在S期后半段复制的BrdU取代DNA高0.010 g/ml。这可能反映了该类似物在S期开始时的优先掺入。这种特殊的掺入模式可能是导致在存在该类似物的情况下，被劳氏肉瘤病毒感染的成纤维细胞生长时观察到的细胞转化受到抑制的原因。
