Lin B L, Makino T, Suekane H, Takayama S, Yoshimura S, Ohno T, Iizuka R
Nihon Sanka Fujinka Gakkai Zasshi. 1982 Oct;34(10):1684-90.
The pituitary gonadotropin response of synthesis and release to two step stimulation by 100 micrograms of LH-RH at 60 minutes interval was studied by radioimmunoassay of LH and FSH in normal menstrual cycling and menopause women and adult males. The blood samples were collected before LH-RH stimulation and 30, 60, 90, 120 minute after the first stimulation, respectively. The value of delta 1 was defined as either higher value at 30 or 60 minute minus the value at 0 minute. The value of delta 2 was defined as either higher value at 90, 120, or 180 minute minus the value of 60 minute. delta 1 Was explained as the release of pituitary gonadotropin and delta 2 standed for the release and synthesis of pituitary gonadotropin. delta 1 delta 2 Ratio was expressed as delta 2/delta 1 100%. If delta 2 was higher than delta 1, subsequently the value of delta 1 delta 2 ratio was larger than 100. When delta 1 was more than delta 2, then the value of delta 1 delta 2 ratio was smaller than 100. In normal menstrual women delta 1 of LH were as Preovulatory phase greater than Follicular phase greater than or equal to Luteal phase, delta 2 were as Preovulatory phase greater than Luteal phase greater than Follicular phase. The value of both delta 1 and delta 2 of FSH were Preovulatory phase greater than Follicular phase greater than Luteal phase. The delta 1 of serum LH of menopause women and adult males were higher whereas delta 2 of both serum LH and FSH of adult males were extremely low in value. delta 1 And delta 2 of serum FSH in menopause women showed higher values than those of others. delta 1 delta 2 Ratio of LH and FSH of both follicular phase and luteal phase were more than 100, indicating dominant pattern of synthesis of gonadotropin. The delta 1 delta 2 ratio of LH at preovulatory phase and of both LH and FSH in menopause women and adult males were smaller than 100, suggesting dominant pattern of release of pituitary gonadotropin. These dates suggest that our LH-RH two step test was more useful to clarify anterior pituitary function in terms of both synthesis and release of gonadotropin.
通过对正常月经周期女性、绝经女性及成年男性进行促黄体生成素（LH）和促卵泡生成素（FSH）的放射免疫测定，研究了垂体促性腺激素在间隔60分钟接受100微克促黄体生成素释放激素（LH - RH）两步刺激后的合成与释放反应。分别在LH - RH刺激前以及首次刺激后的30、60、90、120分钟采集血样。δ1值定义为30分钟或60分钟时的较高值减去0分钟时的值。δ2值定义为90、120或180分钟时的较高值减去60分钟时的值。δ1被解释为垂体促性腺激素的释放，δ2代表垂体促性腺激素的释放与合成。δ1δ2比值表示为δ2/δ1×100%。若δ2高于δ1，则δ1δ2比值大于100。当δ1大于δ2时，δ1δ2比值小于100。在正常月经周期女性中，LH的δ1值为排卵前期大于卵泡期大于或等于黄体期，δ2值为排卵前期大于黄体期大于卵泡期。FSH的δ1和δ2值均为排卵前期大于卵泡期大于黄体期。绝经女性和成年男性血清LH的δ1较高，而成年男性血清LH和FSH的δ2值极低。绝经女性血清FSH的δ1和δ2值高于其他人群。卵泡期和黄体期LH及FSH的δ1δ2比值均大于100，表明促性腺激素合成占主导模式。排卵前期LH以及绝经女性和成年男性LH与FSH的δ1δ2比值小于100，提示垂体促性腺激素释放占主导模式。这些数据表明，我们的LH - RH两步试验在阐明促性腺激素的合成与释放方面，对明确垂体前叶功能更有用。
