Fukuoka K, Makino T, Takahashi M, Lin B L, Suekane H, Yokokura T, Kobayashi J, Iizuka R
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo.
Nihon Sanka Fujinka Gakkai Zasshi. 1988 Feb;40(2):137-44.
To clarify both the releasing function and the self-priming effect of LH-RH on gonadotropins of the anterior pituitary gland, two step administration of 100 micrograms of synthetic LH-RH at a 60 minutes interval (two step LH-RH test) was carried out in 29 women with luteal insufficiency, 10 women in luteal phase just after spontaneous abortion, 12 women in puerperium and 24 women with normal menstrual cycles. Native LH, FSH and their subunits were measured by radioimmunoassay and serum progesterone (P), estradiol (E2) and prolactin (PRL) were also measured before administration of LH-RH. By defining hormone release (1st peak level-0' level) as delta 1, self-priming effect (2nd peak level-60' level) as delta 2 and delta 2/delta 1 as delta 1 delta 2 ratio, in the luteal insufficiency group, especially in the cases with lower of serum P and E2, delta 1 was significantly higher and the delta 1 delta 2 ratio was lower than those in the control group. In the abortion group, delta 1 and delta 2 were similar to those in the control group, indicating rapid recovery of the anterior pituitary function. In the puerperium group (approximately 1 month after delivery), delta 1 was higher and the delta 1 delta 2 ratio was lower than those in the control group. This suggests that the puerperium is more or less similar to the period of luteal insufficiency. The results also indicate that the two step LH-RH test can be one of the useful methods for the study of luteal insufficiency in terms of the pituitary gonadotropin synthesis and release.
为阐明促黄体生成激素释放激素（LH-RH）对垂体前叶促性腺激素的释放功能及自身启动效应，对29例黄体功能不全的女性、10例自然流产后处于黄体期的女性、12例产褥期女性及24例月经周期正常的女性，每隔60分钟分两步给予100微克合成LH-RH（两步LH-RH试验）。在给予LH-RH之前，采用放射免疫分析法测定天然LH、FSH及其亚基，同时测定血清孕酮（P）、雌二醇（E2）和催乳素（PRL）。将激素释放（第1个峰值水平 - 0分钟水平）定义为δ1，自身启动效应（第2个峰值水平 - 60分钟水平）定义为δ2，δ2/δ1定义为δ1δ2比值。在黄体功能不全组，尤其是血清P和E2水平较低的病例中，δ1显著升高，且δ1δ2比值低于对照组。在流产组中，δ1和δ2与对照组相似，表明垂体前叶功能迅速恢复。在产褥期组（产后约1个月），δ1较高，δ1δ2比值低于对照组。这表明产褥期在某种程度上与黄体功能不全期相似。结果还表明，就垂体促性腺激素的合成和释放而言，两步LH-RH试验可成为研究黄体功能不全的有用方法之一。
