Feinstein S I, Altman S
Mol Gen Genet. 1982;187(2):347-53. doi: 10.1007/BF00331142.
Nonsense codon suppressing lysogens of E. coli have been made using phi 80 psu3+-A2 and phi 80 psuoc+-A2, heat-sensitive amber and ochre suppressing derivatives, respectively, of bacteriophage phi 80. The various lysogens selected differ in strength of suppression as well as in heat sensitivity of suppressor function. Heat-resistant derivatives, some still carrying the A2 mutation, can be selected from the heat sensitive parents. Mapping experiments indicate that the phi 80 derivatives integrate at the tyrTV locus, which contains two copies of tRNA1Tyr. The origin of the various suppressor phenotypes appears to be related to the great variety of distinctive recombination events possible either between the incoming tRNA1Tyr gene and the host copies, or among the three copies of this gene in the lysogens.
利用噬菌体φ80的热敏感琥珀型和赭石型抑制衍生物φ80 psu3+-A2和φ80 psuoc+-A2，构建了大肠杆菌的无义密码子抑制溶原菌。所选择的各种溶原菌在抑制强度以及抑制功能的热敏感性方面存在差异。可以从热敏感亲本中筛选出耐热衍生物，其中一些仍携带A2突变。定位实验表明，φ80衍生物整合在tyrTV位点，该位点包含两个tRNA1Tyr拷贝。各种抑制表型的起源似乎与传入的tRNA1Tyr基因与宿主拷贝之间，或溶原菌中该基因的三个拷贝之间可能发生的各种独特重组事件有关。
