Kahn L D
Physiol Chem Phys. 1982;14(1):3-7.
Binding of lectins to microbial cell walls was investigated by fluorimetric titration and Scatchard plot. Data were correlated with agglutinability. Concanavalin A and lectins of wheat germ, soybean, pea, lentil, and peanut were tested against Escherichia coli. Micrococcus luteus, Lactobacillus plantarum, and Bacillus subtilis. In cases where binding occurred, it was either nonspecific or positively cooperative. Agglutination was observed only in those combinations of lectin and microorganism that showed positive cooperative binding, suggesting a definite relation between binding and agglutination. Lectins binding to the same carbohydrate did not necessarily bind to the same microorganism, confirming the complexity of the lectin-receptor bond.
通过荧光滴定和Scatchard图研究了凝集素与微生物细胞壁的结合。数据与凝集性相关。测试了伴刀豆球蛋白A以及小麦胚芽、大豆、豌豆、小扁豆和花生的凝集素对大肠杆菌、藤黄微球菌、植物乳杆菌和枯草芽孢杆菌的作用。在发生结合的情况下，要么是非特异性的，要么是正协同的。仅在那些显示正协同结合的凝集素和微生物组合中观察到凝集现象，这表明结合与凝集之间存在明确的关系。结合相同碳水化合物的凝集素不一定结合相同的微生物，这证实了凝集素-受体键的复杂性。
