Ruzicka T, Printz M P
Agents Actions. 1982 Oct;12(4):527-9. doi: 10.1007/BF01965938.
We investigated the effect of chloroquine (CQ) on the metabolism of exogenous [14C]arachidonic acid by guinea pig skin homogenates. The major cyclooxygenase product formed by the homogenates was PGD2; smaller quantities of PGE2 were formed. CQ suppressed the production of PGD2 and PGE2 in a dose-dependent fashion with an IC50 of 230 microM. This suppression was due to a cyclooxygenase inhibition, since the formation of the prostaglandins from exogenous endoperoxide PGH2 was unaffected. The skin homogenates also exhibited an active lipoxygenase pathway leading to HETE formation. The effect of CQ on the lipoxygenase activity was far less marked than its effect on the cyclooxygenase. CQ is an inhibitor of the cutaneous cyclooxygenase, and this effect may contribute to its anti-inflammatory action in various dermatological disorders.
我们研究了氯喹（CQ）对豚鼠皮肤匀浆中外源性[14C]花生四烯酸代谢的影响。匀浆形成的主要环氧化酶产物是PGD2；形成的PGE2量较少。CQ以剂量依赖性方式抑制PGD2和PGE2的产生，IC50为230微摩尔。这种抑制是由于环氧化酶抑制，因为外源性内过氧化物PGH2形成前列腺素不受影响。皮肤匀浆还表现出导致HETE形成的活跃脂氧合酶途径。CQ对脂氧合酶活性的影响远不如其对环氧化酶的影响明显。CQ是皮肤环氧化酶的抑制剂，这种作用可能有助于其在各种皮肤病中的抗炎作用。
