Kaufmann S H, Simon M M, Hahn H
Infect Immun. 1982 Dec;38(3):907-13. doi: 10.1128/iai.38.3.907-913.1982.
Peritoneal exudate T lymphocytes from Listeria monocytogenes-immune mice in the presence of the homologous antigen (heat-killed L. monocytogenes) and normal macrophages showed L. monocytogenes-specific proliferative responses. Proliferation was inhibited by macrophages from L. monocytogenes- or Corynebacterium parvum-pretreated mice as well as by exogenous prostaglandin E(2). Macrophage-dependent inhibition of T-cell proliferation-at least in part-could be reversed by addition of indomethacin. When selected L. monocytogenes-immune Lyt T-cell subsets were cultured in the presence of inhibitory macrophages, pretreatment with anti-Lyt 1 antiserum plus complement completely abrogated proliferation and pretreatment with anti-Lyt 2 and anti-Lyt 3 antisera plus complement markedly reduced proliferation. However, a mixture (1:1) of the two preselected Lyt T-cell subsets resulted in complete reconstitution of proliferative responses. In contrast, when L. monocytogenes-immune peritoneal exudate T lymphocytes were treated with anti-Lyt antisera plus complement after culture, only treatment with anti-Lyt 1 antiserum plus complement affected proliferation, suggesting regulatory interactions between Lyt 1(+)23(-) and Lyt 1(-)23(+) T cells during in vitro culture which result in proliferation within the Lyt 1(+)23(-) T-cell subset. After rigorous depletion of residual macrophages and in the presence of indomethacin, pretreatment with anti-Lyt 1 antiserum plus complement, but not with anti-Lyt 2 and 3 antisera plus complement, eliminated proliferation. The data presented indicate that interactions between macrophages and Lyt T-cell subsets regulate L. monocytogenes-specific T-cell activation.
在同源抗原（热灭活的单核细胞增生李斯特菌）存在的情况下，来自单核细胞增生李斯特菌免疫小鼠的腹腔渗出液T淋巴细胞与正常巨噬细胞一起时，表现出单核细胞增生李斯特菌特异性增殖反应。来自单核细胞增生李斯特菌或短小棒状杆菌预处理小鼠的巨噬细胞以及外源性前列腺素E(2)可抑制增殖。添加消炎痛可部分逆转巨噬细胞依赖性的T细胞增殖抑制作用。当在抑制性巨噬细胞存在的情况下培养选定的单核细胞增生李斯特菌免疫Lyt T细胞亚群时，用抗Lyt 1抗血清加补体预处理可完全消除增殖，而用抗Lyt 2和抗Lyt 3抗血清加补体预处理则可显著降低增殖。然而，两种预先选定的Lyt T细胞亚群的混合物（1:1）可导致增殖反应完全恢复。相反，当单核细胞增生李斯特菌免疫的腹腔渗出液T淋巴细胞在培养后用抗Lyt抗血清加补体处理时，只有用抗Lyt 1抗血清加补体处理才会影响增殖，这表明在体外培养过程中Lyt 1(+)23(-)和Lyt 1(-)23(+) T细胞之间存在调节相互作用，从而导致Lyt 1(+)23(-) T细胞亚群内的增殖。在严格去除残留巨噬细胞并存在消炎痛的情况下，用抗Lyt 1抗血清加补体预处理可消除增殖，而用抗Lyt 2和3抗血清加补体预处理则不能。所呈现的数据表明，巨噬细胞与Lyt T细胞亚群之间的相互作用调节单核细胞增生李斯特菌特异性T细胞活化。
