骨扫描剂99mTc（Sn）EHDP的制备方法对其在大鼠体内组织分布的影响。

The influence of the method of preparation of the bone scanning agent 99mTc(Sn)EHDP on its tissue distribution in the rat.

作者信息

Van den Brand J A, Das H A, Dekker B G, de Ligny C L, Van den Hamer C J

出版信息

Int J Appl Radiat Isot. 1982 Oct;33(10):917-28. doi: 10.1016/0020-708x(82)90137-5.

Abstract

The tissue distribution in the rat of the bone scanning agent 99mTc(Sn)EHDP is investigated by three methods, viz. by dissecting the animals 3 and 24 h post injection, by scanning the animals with a rectilinear scanner and by whole body autoradiography, particular of micro-sections of the skeleton. The effect of varying the experimental conditions in the preparation of the bone scanning agent (pH and concentrations of Sn(II) and EHDP) is investigated. In some of the experiments additional amounts of Sn(II) and EHDP are added to the reaction mixture after the preparation of the bone scanning agent. In this way the influence of the Sn(II) and EHDP concentrations on the composition of the bone scanning agent, and their influence as such on tissue uptake can be separately investigated. The 99mTc-uptake in bone is compared with the distribution of the 99mTc over the two main products in the reaction mixture, viz. 99mTc(IV)-Sn-EHDP2 and 99mTc(IV)-Sn-EHDP. In addition, the stability in vitro of some of the preparations in urine and plasma and the in vivo binding of the 99mTc to plasma proteins are determined. A procedure for the preparation of the bone scanning agent is recommended.

摘要

采用三种方法研究了骨扫描剂99mTc(Sn)EHDP在大鼠体内的组织分布，即注射后3小时和24小时解剖动物、用直线扫描仪对动物进行扫描以及全身放射自显影，特别是骨骼微切片的放射自显影。研究了在制备骨扫描剂时改变实验条件（pH值以及Sn(II)和EHDP的浓度）的影响。在一些实验中，在制备骨扫描剂后向反应混合物中额外添加Sn(II)和EHDP。通过这种方式，可以分别研究Sn(II)和EHDP浓度对骨扫描剂组成的影响以及它们对组织摄取的影响。将骨中99mTc的摄取与反应混合物中两种主要产物99mTc(IV)-Sn-EHDP2和99mTc(IV)-Sn-EHDP上99mTc的分布进行比较。此外，还测定了一些制剂在尿液和血浆中的体外稳定性以及99mTc在体内与血浆蛋白的结合情况。推荐了一种骨扫描剂的制备方法。