免疫珠酶联免疫吸附测定法测定唾液中免疫球蛋白A：与单向辐射免疫扩散法的比较
Determination of immunoglobulin A in saliva by immunobead enzyme-linked immunosorbent assay: comparison with single radial immunodiffusion.
作者信息
Bratthall D, Ellen R P
出版信息
J Clin Microbiol. 1982 Oct;16(4):766-9. doi: 10.1128/jcm.16.4.766-769.1982.
Abstract
For the quantitative measurement of immunoglobulin A in human saliva, an immunobead enzyme-linked immunosorbent assay was investigated in detail. The method proved to be fast and sensitive, and it gave reproducible results. There was a strong correlation with results obtained by a single radial immunodiffusion method. The immunobead method gave 1.8 times higher values than the single radial immunodiffusion method with a lower correction factor for low-immunoglobulin A saliva and a higher factor for immunoglobulin A-rich saliva.
摘要
为了定量检测人唾液中的免疫球蛋白A，对免疫珠酶联免疫吸附测定法进行了详细研究。该方法被证明快速且灵敏，结果具有可重复性。与单扩散免疫比浊法获得的结果有很强的相关性。免疫珠法得到的值比单扩散免疫比浊法高1.8倍，对于低免疫球蛋白A唾液其校正因子较低，而对于富含免疫球蛋白A的唾液其校正因子较高。
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