Kukain E M, Nikolaeva T A, Khazenson L B
Zh Mikrobiol Epidemiol Immunobiol. 1975 Mar;0(3):56-61.
In comparison with the radial immunodiffusion method, the sensitivity of the indirect hemagglutination test in determining the concentration of immunoglobulins G, M and A in various biological substrates (blood serum, saliva, cerebrospinal fluid, feces) was 35--100 greater. Under the action of proteolytic enzymes immunoglobulin fragmentation led to increase in the concentration of the Ig indices (according to the data of radial immunodiffusion) and produced no effect on the indices of the indirect hemagglutination test; The latter should be used to determine the immunoglobulins in the external secretions characterized by a low content of the given proteins and a marked probability of their proteolytic splitting. In assessing the results of the indirect hemagglutination test it should be remembered that it was less precise and less reproducible than the radial immunodiffusion method.
与放射免疫扩散法相比，间接血凝试验在测定各种生物底物（血清、唾液、脑脊液、粪便）中免疫球蛋白G、M和A浓度时的灵敏度要高35至100倍。在蛋白水解酶的作用下，免疫球蛋白片段化导致免疫球蛋白指数浓度升高（根据放射免疫扩散数据），但对间接血凝试验的指标没有影响；后者应用于测定特定蛋白质含量低且其蛋白水解裂解可能性显著的外分泌液中的免疫球蛋白。在评估间接血凝试验结果时，应记住该方法不如放射免疫扩散法精确和可重复。
