Yasuda N, Kanoh T, Shirakawa S, Uchino H
Leuk Res. 1982;6(5):659-67. doi: 10.1016/0145-2126(82)90082-0.
The presence of ultrastructural distribution of intracellular immunoglobulin (Ig) in leukemic cells from patients with chronic lymphocytic leukemia (CLL) and in normal B cells were investigated by an immunoelectron microscopic technique. Most normal B cells had no intracellular Ig in spite of the presence of surface Ig. However, leukemic cells from 10 to 11 patients with CLL contained intracellular Ig and showed various staining patterns. In eight patients, Ig was present in the perinuclear space (PN), the endoplasmic reticulum (ER) and, if identified, the Golgi complexes. In four patients, most cells had diffuse staining of the cytoplasm. In five patients, Ig was detected in the ER-associated structures or the vesicles, in addition to the PN and ER. These findings suggest that CLL cells have a greater capacity to produce Ig than those of normal B cells and include various clones with distinct staining patterns of intracellular Ig.
采用免疫电子显微镜技术研究了慢性淋巴细胞白血病（CLL）患者白血病细胞及正常B细胞内免疫球蛋白（Ig）的超微结构分布情况。尽管大多数正常B细胞表面存在Ig，但细胞内并无Ig。然而，10至11例CLL患者的白血病细胞含有细胞内Ig，并呈现出各种染色模式。在8例患者中，Ig存在于核周间隙（PN）、内质网（ER）以及（若能识别）高尔基体复合物中。在4例患者中，大多数细胞的细胞质呈弥漫性染色。在5例患者中，除PN和ER外，在ER相关结构或囊泡中也检测到了Ig。这些发现表明，CLL细胞产生Ig的能力比正常B细胞更强，且包含具有不同细胞内Ig染色模式的各种克隆。
