血清和红细胞中叶酸的微量滴定板微生物学测定。
Microbiological assay on microtitre plates of folate in serum and red cells.
作者信息
O'Broin S, Kelleher B
机构信息
Department of Haematology, St James's Hospital, Dublin, Ireland.
出版信息
J Clin Pathol. 1992 Apr;45(4):344-7. doi: 10.1136/jcp.45.4.344.
AIMS
To develop a simple microbiological assay for serum and red cell folates on 96-well microtitre plates, suitable for use in routine clinical diagnosis.
METHODS
Use of a chloramphenicol resistant organism (NCIB 10463) saved time by avoiding aseptic precautions. Use of plate sealers facilitated mixing. Evaluation of assay performance included estimations of folate recovery, assay reproducibility, and response to reduced folate. Results obtained on sera (193) and red cell folates (150) were compared with those obtained using a traditional microbiological assay.
RESULTS
Good recovery of folic acid added to serum and also good interassay and intra-assay precision were obtained with both serum (CV% of less than 5) and red cell folate pools (CV% of less than 5). Equimolar assay responses were obtained with folic acid, 5-formyltetrahydrofolate (L-form), and 5-methyltetrahydrofolate (L-form). The microassay correlated well with a traditional assay for estimation of folate in both serum (n = 193, r = 0.975) and red cells (n = 150, r = 0.96).
CONCLUSIONS
This assay is more compact and less time consuming than the traditional assay. It is extremely economical and is easy to perform in a routine clinical laboratory.
目的
开发一种用于在96孔微量滴定板上检测血清和红细胞叶酸的简单微生物学检测方法，适用于常规临床诊断。
方法
使用耐氯霉素的生物体（NCIB 10463）避免了无菌操作，节省了时间。使用板密封剂便于混合。检测性能评估包括叶酸回收率、检测重现性和对还原型叶酸的反应。将193份血清和150份红细胞叶酸的检测结果与使用传统微生物学检测方法得到的结果进行比较。
结果
血清（CV%小于5）和红细胞叶酸池（CV%小于5）对添加到血清中的叶酸均有良好的回收率，且检测间和检测内精密度良好。叶酸、5-甲酰四氢叶酸（L型）和5-甲基四氢叶酸（L型）获得了等摩尔检测反应。该微量检测法与传统检测法在血清（n = 193，r = 0.975）和红细胞（n = 150，r = 0.96）叶酸估计方面相关性良好。
结论
该检测方法比传统检测方法更紧凑、耗时更少。它极其经济，易于在常规临床实验室中进行。
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