Botes D P, Viljoen C C, Kruger H, Wessels P L, Williams D H
Toxicon. 1982;20(6):1037-42. doi: 10.1016/0041-0101(82)90105-2.
The configuration assignment of the alpha-carbon atom of amino acid residues in four toxin variants from Microcystis aeruginosa have been made by stereospecific enzymic transformations. The relative conformation assignment of the beta-carbon atom of beta-CH3-aspartic acid could be made by comparison of the electrophoretic mobility with literature values reported for the authentic compound. The presence of an N-methyldehydroalanine residue, which, due to elimination of methylamine under hydrolytic conditions, previously escaped detection by conventional means, has been confirmed by identification of N-methylalanine in the hydrolysate after reduction of toxin with sodium borohydride.
通过立体特异性酶促转化确定了铜绿微囊藻四种毒素变体中氨基酸残基α-碳原子的构型。通过将β-CH3-天冬氨酸β-碳原子的电泳迁移率与文献报道的真实化合物的值进行比较，可以确定其相对构象。在硼氢化钠还原毒素后，通过鉴定水解产物中的N-甲基丙氨酸，证实了存在N-甲基脱氢丙氨酸残基，该残基由于在水解条件下消除甲胺，以前通过常规方法未被检测到。
