Schartl M, Barnekow A
Differentiation. 1982;23(2):109-14. doi: 10.1111/j.1432-0436.1982.tb01273.x.
All specimens of Eumetazoa and Parazoa, ranging from mammals, birds, teleosts, sharks, lampreys, amphioxus, insects, down to sponges showed the pp60c-src associated kinase activity, indicating that c-src, which is the cellular homologue of the oncogene v-src of Rous sarcoma virus (RSV) is probably present in all multicellular animals. Protozoa and plants did not show pp60c-src kinase activity. The degree of c-src expression depends on the taxonomic rank of the Eumetazoa tested, and is organ-specific with nervous tissues displaying the highest kinase activities. In the central nervous system of mammals and birds we found a high c-src expression, and in that of the lampreys, amphioxus, and insects the lowest. Unexpectedly, total extracts of sponges showed an amount of pp60c-src kinase activity similar to that of brain cell extracts of mammals and birds. These findings suggest that pp60c-src is a phylogenetic old protein that might have evolved together with the multicellular organisation of Metazoa, and that might be of importance in proliferation and differentiation of nontransformed cells.
所有真后生动物和侧生动物的样本，从哺乳动物、鸟类、硬骨鱼、鲨鱼、七鳃鳗、文昌鱼、昆虫到海绵，都显示出与pp60c-src相关的激酶活性，这表明作为劳氏肉瘤病毒（RSV）癌基因v-src细胞同源物的c-src可能存在于所有多细胞动物中。原生动物和植物未显示出pp60c-src激酶活性。c-src的表达程度取决于所测试真后生动物的分类等级，并且具有器官特异性，神经组织显示出最高的激酶活性。在哺乳动物和鸟类的中枢神经系统中，我们发现c-src的高表达，而在七鳃鳗、文昌鱼和昆虫的中枢神经系统中表达最低。出乎意料的是，海绵的总提取物显示出的pp60c-src激酶活性与哺乳动物和鸟类的脑细胞提取物相似。这些发现表明，pp60c-src是一种在系统发育上古老的蛋白质，可能与后生动物的多细胞组织一起进化，并且可能在未转化细胞的增殖和分化中起重要作用。
