Abe N, Yamaguchi T, Hoshino F, Suzuki F, Ebina T, Ishida N
Gan. 1982 Oct;73(5):811-8.
The antitumor effect of TH69, a lyophilized whole cell preparation of Streptococcus faecalis, was examined in mice using four experimental tumors: Ehrlich carcinoma and sarcoma-180 in DDI mice, MCA tumor in syngeneic C3H mice and Meth-A tumor in syngeneic BALB/c mice. Consecutive intraperitoneal injections of TH69 started one day after the tumor implantation markedly inhibited the growth of the two allogeneic ascites tumors and two syngeneic solid tumors. However, even with the most sensitive Ehrlich ascites tumor, TH69 was ineffective when the tumor cell inoculum was more than 2 X 10(6) cells per mouse. Neither oral nor subcutaneous administrations of TH69 produced significant antitumor effects. After a single intraperitoneal administration of TH69 (300 mg/kg) to mice, interferon was induced in the serum with a titer of 220 U/ml at 14 hr after administration. As TH69 has no direct cytotoxic activity against Ehrlich tumor cells, the antitumor effect of TH69 in mice must be ascribed to some immunopotentiating activity including interferon induction.
使用四种实验性肿瘤在小鼠中检测了粪肠球菌冻干全细胞制剂TH69的抗肿瘤作用：DDI小鼠中的艾氏癌和肉瘤180、同基因C3H小鼠中的MCA肿瘤以及同基因BALB/c小鼠中的Meth-A肿瘤。在肿瘤植入后一天开始连续腹腔注射TH69，显著抑制了两种同种异体腹水肿瘤和两种同基因实体肿瘤的生长。然而，即使对于最敏感的艾氏腹水肿瘤，当每只小鼠的肿瘤细胞接种量超过2×10⁶个细胞时，TH69也无效。口服或皮下给予TH69均未产生显著的抗肿瘤作用。给小鼠单次腹腔注射TH69（300mg/kg）后，给药后14小时血清中诱导出干扰素，效价为220U/ml。由于TH69对艾氏肿瘤细胞没有直接的细胞毒活性，TH69在小鼠中的抗肿瘤作用一定归因于包括干扰素诱导在内的一些免疫增强活性。
