Bajpai P, Agrawala P K, Vishwanathan L
Can J Microbiol. 1982 Dec;28(12):1340-6. doi: 10.1139/m82-200.
Efforts have been made to develop a simple chemically defined resuspended mycelial system which may be used for carrying out fundamental studies regarding the mechanism of kojic acid biosynthesis. As a first step. it was found that mycelia grown in yeast extract sucrose (YES) medium and resuspended in YES medium or in 0.2 M phosphate buffer, pH 6.5, supplemented with 20% glucose or sucrose produced kojic acid almost to the same extent as in the case of growth medium. No kojic acid was formed if buffers or media used for resuspension lacked carbohydrate. Intact mycelia preincubated in buffer alone for 7 days and 3-week-old mycelia could still form kojic acid in large amounts if resuspended in buffer containing glucose. The amount of kojic acid produced by the intact mycelia was found to be more than that produced by the disrupted mycelia. In contrast with static resuspension studies, when Aspergillus flavus mycelia were resuspended in flasks placed on a rotary shaker, much smaller amounts of kojic acid were synthesized.
人们已努力开发一种简单的化学成分明确的重悬菌丝体系统，该系统可用于开展关于曲酸生物合成机制的基础研究。第一步，发现生长在酵母提取物蔗糖（YES）培养基中并重悬于YES培养基或补充有20%葡萄糖或蔗糖的pH 6.5的0.2 M磷酸盐缓冲液中的菌丝体产生曲酸的程度几乎与在生长培养基中的情况相同。如果用于重悬的缓冲液或培养基缺乏碳水化合物，则不会形成曲酸。单独在缓冲液中预孵育7天的完整菌丝体以及3周龄的菌丝体，如果重悬于含有葡萄糖的缓冲液中，仍能大量形成曲酸。发现完整菌丝体产生的曲酸量多于破碎菌丝体产生的曲酸量。与静态重悬研究相反，当黄曲霉菌丝体重悬于置于旋转摇床上的烧瓶中时，合成的曲酸量要少得多。
