Watanabe F, Tsubota N, Kobayashi Y, Miyata O, Ninomiya I, Miyai K
Steroids. 1982 Oct;40(4):393-401. doi: 10.1016/0039-128x(82)90017-4.
Solid phase fluoroimmunoassay of serum 11-deoxycortisol (17,21-dihydroxy-4-pregnene-3,20-dione) was established using fluorescein isothiocyanate-labelled 11-deoxycortisol and anti-11-deoxycortisol antibody-conjugated polyacrylamide beads. 21-Amino-17-hydroxyprogesterone (21-amino-17-hydroxy-4-pregnene-3,20-dione) was synthesized as a useful derivative for preparing the fluorescent dye conjugate. Serum 11-deoxycortisol was measured with this assay system after extraction and purification by Sephadex LH-20 column chromatography. The minimal amount of 11-deoxycortisol detected was 40 pg/tube and the measurable range was from 0.04 to 5.0 microgram/dl. Intra- and inter-assay coefficients of variation were 8.3% (n=6) and 9.8% (n=5), respectively. 11-deoxycortisol values determined by the present assay correlated well with those determined by radioimmunoassay. The present assay is particularly suitable for estimating the conditions of the pituitary and adrenocortical functions.
使用异硫氰酸荧光素标记的11-脱氧皮质醇和抗11-脱氧皮质醇抗体结合的聚丙烯酰胺珠建立了血清11-脱氧皮质醇（17,21-二羟基-4-孕烯-3,20-二酮）的固相荧光免疫测定法。合成了21-氨基-17-羟孕酮（21-氨基-17-羟基-4-孕烯-3,20-二酮）作为制备荧光染料结合物的有用衍生物。通过Sephadex LH-20柱色谱法提取和纯化后，用该测定系统测量血清11-脱氧皮质醇。检测到的11-脱氧皮质醇的最小量为40 pg/管，可测量范围为0.04至5.0微克/分升。批内和批间变异系数分别为8.3%（n=6）和9.8%（n=5）。本测定法测定的11-脱氧皮质醇值与放射免疫测定法测定的值相关性良好。本测定法特别适用于评估垂体和肾上腺皮质功能的状况。
