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人表皮短期器官培养中的DNA复制。阿非科林的抑制作用以及DNA聚合酶α、β和γ的检测。

DNA replication in short-time organ cultures of human epidermis. Inhibition by aphidicolin, and detection of DNA polymerases alpha, beta, and gamma.

作者信息

Krokan H, Volden G

出版信息

Arch Dermatol Res. 1982;273(1-2):97-101. doi: 10.1007/BF00509032.

DOI:10.1007/BF00509032
PMID:6820927
Abstract

Human epidermis uncontaminated by fibroblasts was isolated by a suction blister method. DNA synthesis in short-time organ cultures of isolated epidermis was strongly inhibited by aphidicolin, suggesting that DNA polymerase alpha is involved in DNA replication in human epidermis. On the basis of their responses to inhibitors, primer-template requirements, and chromatographic properties, DNA polymerases alpha, beta, and gamma were all identified in epidermal extracts.

摘要

通过水疱抽吸法分离出未受成纤维细胞污染的人表皮。阿非科林强烈抑制分离表皮短期器官培养中的DNA合成，这表明DNA聚合酶α参与人表皮中的DNA复制。根据它们对抑制剂的反应、引物-模板需求和色谱特性，在表皮提取物中鉴定出了DNA聚合酶α、β和γ。

相似文献

1
DNA replication in short-time organ cultures of human epidermis. Inhibition by aphidicolin, and detection of DNA polymerases alpha, beta, and gamma.人表皮短期器官培养中的DNA复制。阿非科林的抑制作用以及DNA聚合酶α、β和γ的检测。
Arch Dermatol Res. 1982;273(1-2):97-101. doi: 10.1007/BF00509032.
2
Differential sensitivity to aphidicolin of replicative DNA synthesis and ultraviolet-induced unscheduled DNA synthesis in vivo in mammalian cells.
Acta Med Okayama. 1984 Jun;38(3):227-37. doi: 10.18926/AMO/30354.
3
Involvement of eucaryotic deoxyribonucleic acid polymerases alpha and gamma in the replication of cellular and viral deoxyribonucleic acid.真核生物脱氧核糖核酸聚合酶α和γ参与细胞和病毒脱氧核糖核酸的复制。
Biochemistry. 1979 Oct 2;18(20):4431-43. doi: 10.1021/bi00587a025.
4
Control of cell division by aphidicolin without adverse effects upon resting cells.使用阿非迪霉素控制细胞分裂，且对静止细胞无不良影响。
Arzneimittelforschung. 1985;35(7):1108-16.
5
Aphidicolin inhibition of the production of replicative-form DNA during bovine parvovirus infection.阿非科林对牛细小病毒感染期间复制型DNA产生的抑制作用。
J Virol. 1984 Mar;49(3):652-7. doi: 10.1128/JVI.49.3.652-657.1984.
6
Specific inhibitors of eukaryotic DNA synthesis and DNA polymerase alpha, 3-deoxyaphidicolin and aphidicolin-17-monoacetate.真核生物DNA合成及DNA聚合酶α的特异性抑制剂，3-脱氧杀稻瘟菌素和杀稻瘟菌素-17-单乙酸酯。
Nucleic Acids Res. 1983 Feb 25;11(4):1197-209. doi: 10.1093/nar/11.4.1197.
7
DNA repair in nuclei isolated from HeLa cells.从HeLa细胞中分离出的细胞核中的DNA修复
Basic Life Sci. 1980;15:357-65. doi: 10.1007/978-1-4684-3842-0_24.
8
The effect of aphidicolin on adenovirus DNA synthesis.阿非科林对腺病毒DNA合成的影响。
Nucleic Acids Res. 1979 Jul 25;6(10):3369-86. doi: 10.1093/nar/6.10.3369.
9
Terminal differentiation of human erythroleukemia cell line K562 induced by aphidicolin.
Exp Cell Res. 1990 Nov;191(1):45-50. doi: 10.1016/0014-4827(90)90033-7.
10
Effect of aphidicolin on de novo DNA synthesis, DNA repair and cytotoxicity in gamma-irradiated human fibroblasts. Implications for the enhanced radiosensitivity in ataxia telangiectasia.阿非科林对经γ射线照射的人成纤维细胞中DNA从头合成、DNA修复及细胞毒性的影响。共济失调毛细血管扩张症中放射敏感性增强的意义。
Biochim Biophys Acta. 1983 Jan 20;739(1):17-26. doi: 10.1016/0167-4781(83)90039-8.

本文引用的文献

1
Autoradiographic studies of the incorporation of thymidine-H3 into deoxyribonucleic acid in the skin of young rats.用氚标记胸腺嘧啶核苷掺入幼鼠皮肤脱氧核糖核酸的放射自显影研究。
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2
Flow cytometry (FCM) of human epidermal cells. A preparation method for epidermal cells and demonstration of circadian variations in the proportion of S-phase cells.人表皮细胞的流式细胞术（FCM）。一种表皮细胞的制备方法及S期细胞比例昼夜变化的证明。
Arch Dermatol Res. 1980;269(2):137-45. doi: 10.1007/BF00406533.
3
Aphidicolin inhibits DNA synthesis by DNA polymerase alpha and isolated nuclei by a similar mechanism.
阿非科林通过抑制DNA聚合酶α来抑制DNA合成，并且通过类似机制抑制分离的细胞核（的相关活动）。
Nucleic Acids Res. 1981 Sep 25;9(18):4709-19. doi: 10.1093/nar/9.18.4709.
4
New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha.通过一种不同寻常的DNA聚合酶α抑制剂——阿非科林揭示的真核生物DNA复制生物化学新观点。
Cell. 1981 Mar;23(3):647-8. doi: 10.1016/0092-8674(81)90426-8.
5
Synchronization of HeLa cell cultures by inhibition of DNA polymerase alpha with aphidicolin.通过用阿非迪霉素抑制DNA聚合酶α来同步化HeLa细胞培养物。
Nucleic Acids Res. 1980 Jan 25;8(2):377-87. doi: 10.1093/nar/8.2.377.
6
Lack of mutagenicity and metabolic inactivation of aphidicolin by rat liver microsomes.
Biochem Biophys Res Commun. 1980 Apr 29;93(4):1094-103. doi: 10.1016/0006-291x(80)90601-4.
7
Aphidicolin allows a rapid and simple evaluation of DNA-repair synthesis in damaged human cells.阿非科林可对受损人类细胞中的DNA修复合成进行快速且简单的评估。
Mutat Res. 1980 May;70(3):389-94. doi: 10.1016/0027-5107(80)90029-9.
8
DNA polymerase gamma, cytochrome c oxidase and mitochondrial integrity in rabbit spleen lymphocytes stimulated with concanavalin A.
Exp Cell Res. 1980 Jun;127(2):269-76. doi: 10.1016/0014-4827(80)90432-2.
9
Dermo-epidermal separation with suction. Electron microscopic and histochemical study of initial events of blistering on human skin.负压引起的真皮-表皮分离。人皮肤水疱形成初始事件的电子显微镜和组织化学研究。
J Invest Dermatol. 1967 May;48(5):466-77.
10
Tritiated thymidine labelling of normal human epidermal cell nuclei. A comparison, in the same subjects, of in vivo and in vitro techniques.
Br J Dermatol. 1969 Aug;81(8):603-16. doi: 10.1111/j.1365-2133.1969.tb16043.x.