• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过染色体原位杂交确定人类主要组织相容性复合体中基因座的定位

Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization.

作者信息

Morton C C, Kirsch I R, Nance W E, Evans G A, Korman A J, Strominger J L

出版信息

Proc Natl Acad Sci U S A. 1984 May;81(9):2816-20. doi: 10.1073/pnas.81.9.2816.

DOI:10.1073/pnas.81.9.2816
PMID:6585830
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC345161/
Abstract

We have determined the localization and orientation of two genetic probes within the human major histocompatibility complex by chromosomal in situ hybridization. Our data indicate that a cloned genomic probe cross-hybridizing to HLA-A, -B, and -C heavy chain loci is homologous to sequences located on chromosome 6 at band p21.3 while a subclone of the genomic HLA-DR alpha-chain gene corresponding to the nonpolymorphic p34 protein is homologous to sequences in band 6p21.1. Our data suggest that this technique may permit the estimation of map distances between linked gene loci, assuming a uniform frequency of map units in the human genome. The relative positions of these genes was confirmed in a mother and son carrying a chromosome rearrangement involving 6p and 14p in which the sequences hybridizing to a DR alpha-chain genomic clone were found at the distal end of the 6p--chromosome [der(6)] while the sequences hybridizing to the HLA-A, -B, -C alpha-chain probe were found in the 14p+ chromosome [der(14)].

摘要

我们通过染色体原位杂交确定了两种基因探针在人类主要组织相容性复合体中的定位和方向。我们的数据表明，一个与HLA - A、- B和 - C重链基因座交叉杂交的克隆基因组探针与位于6号染色体p21.3带的序列同源，而基因组HLA - DRα链基因对应于非多态性p34蛋白的一个亚克隆与6p21.1带中的序列同源。我们的数据表明，假设人类基因组中图谱单位的频率均匀，该技术可能允许估计连锁基因座之间的图谱距离。这些基因的相对位置在一位携带涉及6p和14p染色体重排的母亲和儿子中得到证实，其中与DRα链基因组克隆杂交的序列在6p - 染色体[der(6)]的远端被发现，而与HLA - A、- B、- Cα链探针杂交的序列在14p + 染色体[der(14)]中被发现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aec7/345161/64d155549f93/pnas00610-0226-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aec7/345161/815d2deaa833/pnas00610-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aec7/345161/64d155549f93/pnas00610-0226-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aec7/345161/815d2deaa833/pnas00610-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aec7/345161/64d155549f93/pnas00610-0226-a.jpg

相似文献

1
Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization.通过染色体原位杂交确定人类主要组织相容性复合体中基因座的定位
Proc Natl Acad Sci U S A. 1984 May;81(9):2816-20. doi: 10.1073/pnas.81.9.2816.
2
A minimum of four human class II alpha-chain genes are encoded in the HLA region of chromosome 6.至少四个人类II类α链基因在6号染色体的HLA区域编码。
Nature. 1983;304(5922):174-7. doi: 10.1038/304174a0.
3
Mapping of the genes encoding the HLA-DR alpha chain and the HLA-related antigens to a chromosome 6 deletion by using genomic blotting.利用基因组印迹法将编码HLA - DRα链和HLA相关抗原的基因定位到6号染色体缺失区域。
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2300-4. doi: 10.1073/pnas.80.8.2300.
4
The major histocompatibility complex of tassel-eared squirrels. I. Genetic diversity associated with Kaibab squirrels.缨耳松鼠的主要组织相容性复合体。I. 与凯巴布松鼠相关的遗传多样性。
Immunogenetics. 1986;24(4):230-41. doi: 10.1007/BF00364527.
5
Polymorphic class II sequences linked to the rat major histocompatibility complex (RT1) homologous to human DR and DQ sequences.与大鼠主要组织相容性复合体（RT1）相关的多态性II类序列，与人DR和DQ序列同源。
J Immunol. 1985 Aug;135(2):1450-5.
6
Localization of the human MHC-linked complement genes between HLA-B and HLA-DR by using HLA mutant cell lines.利用HLA突变细胞系将人类MHC连锁补体基因定位在HLA - B和HLA - DR之间。
J Immunol. 1985 Jan;134(1):641-3.
7
The human major histocompatibility complex. Genes and proteins.
Ann N Y Acad Sci. 1985;458:262-8. doi: 10.1111/j.1749-6632.1985.tb14611.x.
8
Recent developments in HLA typing.人类白细胞抗原分型的最新进展。
Prog Clin Biol Res. 1982;88:125-42.
9
Deletion mapping of HLA and chromosome 6p genes.HLA与6号染色体短臂基因的缺失图谱分析。
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3741-5. doi: 10.1073/pnas.82.11.3741.
10
Molecular genetics of the human major histocompatibility complex.人类主要组织相容性复合体的分子遗传学
Adv Hum Genet. 1986;15:197-247. doi: 10.1007/978-1-4615-8356-1_4.

引用本文的文献

1
Sarcoma Common MHC-I Haplotype Restricts Tumor-Specific CD8+ T Cell Response.肉瘤常见的主要组织相容性复合体I类单倍型限制肿瘤特异性CD8 + T细胞反应。
Cancers (Basel). 2022 Jul 14;14(14):3414. doi: 10.3390/cancers14143414.
2
Pas de deux: natural killer receptors and MHC class I ligands in primates.二重奏：灵长类动物中的自然杀伤细胞受体和 MHC Ⅰ类配体。
Curr Genomics. 2007 Mar;8(1):51-7. doi: 10.2174/138920207780076974.
3
The human genes for hemophilia A and hemophilia B flank the X chromosome fragile site at Xq27.3.血友病A和血友病B的人类基因位于X染色体Xq27.3处的脆性位点两侧。

本文引用的文献

1
Chromosome preparations of leukocytes cultured from human peripheral blood.从人外周血培养的白细胞的染色体标本制备。
Exp Cell Res. 1960 Sep;20:613-6. doi: 10.1016/0014-4827(60)90138-5.
2
The identification of human chromosomes by quinacrine fluorescence after hybridisation in situ.原位杂交后用喹吖因荧光法鉴定人类染色体。
Hum Genet. 1980;53(3):371-3. doi: 10.1007/BF00287058.
3
Chromosomes and cancer: new nomenclature and future directions.染色体与癌症：新命名法及未来方向
EMBO J. 1985 Mar;4(3):725-9. doi: 10.1002/j.1460-2075.1985.tb03689.x.
4
Refinement of HLA gene mapping with induced B-cell line mutants.利用诱导B细胞系突变体对HLA基因进行定位优化。
Immunogenetics. 1985;21(3):277-91. doi: 10.1007/BF00375380.
5
Diversity in G + C content at the third position of codons in vertebrate genes and its cause.脊椎动物基因密码子第三位的G + C含量多样性及其成因。
Nucleic Acids Res. 1986 Aug 26;14(16):6345-55. doi: 10.1093/nar/14.16.6345.
6
The role of somatic cell genetics in human gene mapping.体细胞遗传学在人类基因定位中的作用。
Experientia. 1986 Oct 15;42(10):1128-37. doi: 10.1007/BF01941287.
7
Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex.肿瘤坏死因子α和β的基因与人类主要组织相容性复合体相关联。
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8699-702. doi: 10.1073/pnas.83.22.8699.
8
T-cell clones and T-cell receptors.T细胞克隆与T细胞受体。
Microbiol Rev. 1986 Mar;50(1):50-69. doi: 10.1128/mr.50.1.50-69.1986.
9
The chromosome region containing the highly polymorphic HLA class I genes displays limited large scale variability in the human population.包含高度多态性的HLA I类基因的染色体区域在人类群体中表现出有限的大规模变异性。
EMBO J. 1988 Feb;7(2):395-400. doi: 10.1002/j.1460-2075.1988.tb02826.x.
10
Linkage map of the human major histocompatibility complex including the tumor necrosis factor genes.包含肿瘤坏死因子基因的人类主要组织相容性复合体的连锁图谱。
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8535-9. doi: 10.1073/pnas.84.23.8535.
Hum Pathol. 1981 Jun;12(6):494-503. doi: 10.1016/s0046-8177(81)80063-9.
4
Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization.通过原位杂交对G带人类染色体单拷贝DNA序列进行定位
Chromosoma. 1981;83(3):431-9. doi: 10.1007/BF00327364.
5
Partial trisomy 6p: 46,XX, -10, der(10),t(6;10) (p22;q26)pat and HLA localisation.6号染色体短臂部分三体：46,XX, -10, 10号染色体衍生染色体，t(6;10) (p22;q26)父源及HLA定位
J Med Genet. 1981 Jun;18(3):231-4. doi: 10.1136/jmg.18.3.231.
6
Report of the committee on the genetic constitution of chromosome 6. Oslo Conference (1981): Sixth International Workshop on Human Gene Mapping.关于6号染色体遗传构成的委员会报告。奥斯陆会议（1981年）：第六届国际人类基因定位研讨会。
Cytogenet Cell Genet. 1982;32(1-4):130-43. doi: 10.1159/000131693.
7
Chromosomal localization of the human placental lactogen-growth hormone gene cluster to 17q22-24.人胎盘催乳素-生长激素基因簇在染色体上定位于17q22 - 24。
Am J Hum Genet. 1982 Mar;34(2):227-34.
8
Localization of the human insulin gene to the distal end of the short arm of chromosome 11.人类胰岛素基因定位于11号染色体短臂的末端。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4458-60. doi: 10.1073/pnas.78.7.4458.
9
Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules.主要组织相容性抗原：人类（HLA - A、- B、- C）和小鼠（H - 2K、H - 2D）I类分子。
Cell. 1981 May;24(2):287-99. doi: 10.1016/0092-8674(81)90318-4.
10
A processed human immunoglobulin epsilon gene has moved to chromosome 9.一个经过加工的人类免疫球蛋白ε基因已转移至9号染色体。
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5956-60. doi: 10.1073/pnas.79.19.5956.