Muñoz M A, Balón M, Fernandez C
Clin Chem. 1983 Feb;29(2):372-4.
Estimation of inorganic phosphate in serum based on the formation of "molybdenum blue" may be simplified by eliminating the reduction stage. The yellow complex formed by the reaction of phosphate with molybdate in an acid medium is measured at 390 nm. The yellow complex behaves in accordance with Beer's law over a wide concentration range (to at least 80 mg of P per liter in the initial sample); its molar absorptivity at 390 nm is 2.51 X 10(3) L mol-1 cm-1. Sensitivity is increased in the presence of a detergent (triethanolamine lauryl sulfate), which is also used to dissolve the proteins. Because only one reagent is used (a stable combination of, per liter, 20 mmol of sodium molybdate, 82 mmol of nitric acid, and 100 mg of the detergent), the method is simple and rapid. We describe a manual procedure and an automated one. Within-run precision was 1.9%, and day-to-day precision less than 7%. Results by the automated method compare favorably (r = 0.96) with those obtained by Drewes's method (Clin Chim Acta 39: 81-88, 1972).
基于“钼蓝”形成来估算血清中的无机磷酸盐，可通过省去还原步骤而简化。在酸性介质中，磷酸盐与钼酸盐反应形成的黄色络合物于390nm处进行测定。该黄色络合物在较宽的浓度范围内（初始样品中至少每升含80mg的磷）符合比尔定律；其在390nm处的摩尔吸光系数为2.51×10³L·mol⁻¹·cm⁻¹。在存在去污剂（月桂醇硫酸三乙醇胺）的情况下灵敏度会提高，该去污剂也用于溶解蛋白质。由于仅使用一种试剂（每升含有20mmol钼酸钠、82mmol硝酸和100mg去污剂的稳定组合），该方法简单且快速。我们描述了一种手动操作方法和一种自动操作方法。批内精密度为1.9%，日间精密度小于7%。自动方法所得结果与Drewes方法（《临床化学学报》39: 81 - 88, 1972）所得结果相比具有良好的相关性（r = 0.96）。
