Wingard L B
Fed Proc. 1983 Feb;42(2):288-91.
Immobilized enzymes are useful in the development of techniques for analytical chemistry measurements, primarily because of the specificity of enzymes for given substrates. In the case of glucose an in vivo assay is needed for closed loop operation of an artificial pancreas insulin delivery system. Several enzymatic methods are available for in vitro determinations of glucose, but only two or three of these methods hold any promise of adaptation for in vivo use. The most promising enzymatic approaches include the use of immobilized glucose oxidase in conjunction with 1) an oxygen electrode to give an amperometric output or 2) a reference electrode to give a potentiometric output. The use of glucose oxidase or other D-glucose-specific enzymes in conjunction with sensitive thermistors to measure the heat of reaction may also be a feasible method. The two most promising approaches are described in terms of their feasibility for continuous measurement of blood glucose levels in vivo without the necessity of withdrawing body fluids for the assay.
固定化酶在分析化学测量技术的发展中很有用，主要是因为酶对特定底物具有特异性。就葡萄糖而言，人工胰腺胰岛素输送系统的闭环操作需要进行体内测定。有几种酶法可用于体外葡萄糖测定，但其中只有两三种方法有望适用于体内使用。最有前景的酶法包括将固定化葡萄糖氧化酶与以下两种方法结合使用：1）氧电极以产生安培输出，或2）参比电极以产生电位输出。使用葡萄糖氧化酶或其他D-葡萄糖特异性酶结合灵敏热敏电阻来测量反应热也可能是一种可行的方法。本文将介绍两种最有前景的方法在体内连续测量血糖水平的可行性，而无需抽取体液进行检测。
