Neale E A, Oertel W H, Bowers L M, Weise V K
J Neurosci. 1983 Feb;3(2):376-82. doi: 10.1523/JNEUROSCI.03-02-00376.1983.
In order to evaluate the reliability of high affinity [3H]GABA accumulation as a marker for GABAergic neurons, murine cerebral cortical neurons were studied in dissociated cell culture. Cultures which had been incubated in [3H]GABA were stained immunohistochemically for the GABA-synthesizing enzyme, glutamate decarboxylase, fixed with paraformaldehyde, and subsequently processed for radioautography. In mature cultures, there was an 84 to 94% correlation between the presence of the enzyme and [3H]GABA uptake within the same cortical neurons. These data provide direct evidence that those neurons which synthesize GABA are the same neurons which are labeled by high affinity [3H]GABA uptake.
为了评估高亲和力[³H]GABA积累作为GABA能神经元标志物的可靠性，在解离细胞培养中研究了小鼠大脑皮质神经元。用[³H]GABA孵育后的培养物进行免疫组织化学染色，检测GABA合成酶——谷氨酸脱羧酶，用多聚甲醛固定，随后进行放射自显影处理。在成熟培养物中，同一皮质神经元内该酶的存在与[³H]GABA摄取之间存在84%至94%的相关性。这些数据提供了直接证据，表明合成GABA的神经元与通过高亲和力[³H]GABA摄取标记的神经元是相同的神经元。
