Behne D, Wolters W
J Nutr. 1983 Feb;113(2):456-61. doi: 10.1093/jn/113.2.456.
The selenium content was determined in the adrenals, brain, erythrocytes, femur, hair, heart, kidneys, lungs, muscle, pancreas, plasma, spleen, testes, and thymus of rats, which had been fed a commercial rat diet containing 0.3 mg Se/kg diet. In the plasma, the erythrocytes, and the soluble fraction of the tissues (with the exception of femur and hair) the activity of the glutathione peroxidase (GSH-Px) was measured, using both hydrogen peroxide and t-butyl hydroperoxide as substrates. From the masses of the tissues and the values for the selenium content and the GSH-Px activity, the distribution of the element and the enzyme in the body was calculated. For selenium the main pools were the muscle and the liver, and for the GSH-Px, the liver and the erythrocytes. By comparing the selenium content and the GSH-Px activity the percentage of the tissue selenium, which was bound to the enzyme in the soluble tissue fraction, was estimated. This percentage differed considerably from tissue to tissue, the highest value being found in the erythrocytes and the smallest in the testes. According to this estimation the majority of the selenium in the rat is not contained in the GSH-Px but in other compounds.
测定了喂食含0.3毫克硒/千克饲料的市售大鼠饲料的大鼠的肾上腺、脑、红细胞、股骨、毛发、心脏、肾脏、肺、肌肉、胰腺、血浆、脾脏、睾丸和胸腺中的硒含量。在血浆、红细胞以及组织的可溶部分（股骨和毛发除外）中，以过氧化氢和叔丁基过氧化氢作为底物，测定了谷胱甘肽过氧化物酶（GSH-Px）的活性。根据组织质量、硒含量和GSH-Px活性值，计算了元素和酶在体内的分布。对于硒来说，主要蓄积部位是肌肉和肝脏；对于GSH-Px来说，是肝脏和红细胞。通过比较硒含量和GSH-Px活性，估算了可溶组织部分中与该酶结合的组织硒的百分比。该百分比在不同组织间差异很大，在红细胞中最高，在睾丸中最小。根据这一估算结果，大鼠体内的大部分硒并非存在于GSH-Px中，而是存在于其他化合物中。
