Heise E R, Biegel A A, MacQueen M
Transplantation. 1982 Mar;33(3):233-6. doi: 10.1097/00007890-198203000-00005.
Five years of experience with cell exchanges between laboratories comprising the Southeastern Organ Procurement Foundation (SEOPF) were analyzed in order to evaluate progress in HLA typing proficiency. The results of the analysis of 15 cell exchanges involving a total of 60 cells indicate that the average detection rate was 95% or greater for HLA-A1, 2, 3, 9, 10, 11, 28, and 29; B7, 8, 12, 13, 14, 15, 17, 27, and 40. HLA-A locus antigens were detected more often (85.8%) than B locus antigens (80.9%). False negative results were more frequent than false positive antigen assignments. Errors in antigen assignments tended to be nonrandomly distributed for certain antigens belonging to cross-reacting groups but usually were randomly distributed for well defined antigens. During the period of the study, SEOPF laboratories demonstrated improved proficiency in the identification of most HLA-A, B, and C provisional (w) specificities. The results demonstrate the benefits of interlaboratory proficiency testing and indicate that cell exchanges can be carried out successfully on a regional basis.
为评估人类白细胞抗原（HLA）分型熟练度的进展情况，对东南器官采购基金会（SEOPF）下属各实验室间为期五年的细胞交换经验进行了分析。对总共涉及60个细胞的15次细胞交换的分析结果表明，对于HLA - A1、2、3、9、10、11、28和29；B7、8、12、13、14、15、17、27和40，平均检测率达到95%或更高。HLA - A位点抗原的检测频率（85.8%）高于B位点抗原（80.9%）。假阴性结果比抗原指定错误的假阳性更为常见。对于某些属于交叉反应组的抗原，抗原指定错误往往呈非随机分布，但对于明确界定的抗原，通常呈随机分布。在研究期间，SEOPF各实验室在识别大多数HLA - A、B和C临时（w）特异性方面表现出熟练度的提高。结果证明了实验室间熟练度测试的益处，并表明细胞交换可以在区域基础上成功进行。
