荧光光漂白恢复实验的理论分析
Theoretical analysis of fluorescence photobleaching recovery experiments.
作者信息
Soumpasis D M
出版信息
Biophys J. 1983 Jan;41(1):95-7. doi: 10.1016/S0006-3495(83)84410-5.
Abstract
We derive an exact closed formula for the fluorescence recovery curve measured in fluorescence photobleaching recovery experiments employing uniform circular laser beams. In contrast to the expression used currently, this result is very simple and free of mathematical drawbacks, thus facilitating the quantitative analysis of experimental data.
摘要
我们推导出了在使用均匀圆形激光束的荧光漂白恢复实验中测量的荧光恢复曲线的精确封闭公式。与目前使用的表达式不同，这个结果非常简单且没有数学缺陷，从而便于对实验数据进行定量分析。
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本文引用的文献
1
Comparative lateral diffusion of fluorescent lipid analogues in phospholipid multibilayers.荧光脂质类似物在磷脂多层膜中的横向扩散比较
Biochemistry. 1980 Dec 23;19(26):6050-7. doi: 10.1021/bi00567a016.
2
Translational mobility of glycophorin in bilayer membranes of dimyristoylphosphatidylcholine.血型糖蛋白在二肉豆蔻酰磷脂酰胆碱双层膜中的平移流动性。
Biochemistry. 1981 Mar 3;20(5):1392-6. doi: 10.1021/bi00508a055.
3
Dynamics of fluorescence marker concentration as a probe of mobility.作为迁移率探针的荧光标记物浓度动态变化
Biophys J. 1976 Nov;16(11):1315-29. doi: 10.1016/S0006-3495(76)85776-1.
4
Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.通过荧光光漂白恢复动力学分析进行迁移率测量。
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5
Determination of molecular motion in membranes using periodic pattern photobleaching.利用周期性图案光漂白法测定膜中的分子运动
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Fluorescence redistribution after photobleaching. A new multipoint analysis of membrane translational dynamics.光漂白后的荧光再分布。一种新的膜转移动力学多点分析方法。
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