犬纹状体可溶性和天然膜制剂中[3H] -舒必利结合位点某些特性的比较。
A comparison of some of the properties of the [3H]-sulpiride binding site in soluble and native membrane preparations of dog striatum.
作者信息
Templeton W W, Woodruff G N
出版信息
Br J Pharmacol. 1983 Jan;78(1):5-7. doi: 10.1111/j.1476-5381.1983.tb09355.x.
Abstract
[3H]-sulpiride binding sites in dog striatum have been solubilised using CHAPS detergent. Solubilised sites were similar to those in native membrane preparations in terms of Bmax, Kd and sensitivity to dopamine and dopamine receptor antagonists. Binding of [3H]-sulpiride to the solubilised binding site was dependent upon the presence of sodium ions and was inactivated by the sulphydryl group reagent N-ethylmaleimide.
摘要
已使用CHAPS去污剂溶解犬纹状体中的[3H] - 舒必利结合位点。就最大结合容量（Bmax）、解离常数（Kd）以及对多巴胺和多巴胺受体拮抗剂的敏感性而言，溶解后的位点与天然膜制剂中的位点相似。[3H] - 舒必利与溶解后的结合位点的结合依赖于钠离子的存在，并被巯基试剂N - 乙基马来酰亚胺灭活。
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