Bianchi V, Zantedeschi A, Ronchese F, Levis A G
Cancer Lett. 1983 Feb;18(1):21-7. doi: 10.1016/0304-3835(83)90113-1.
The induction of DNA repair synthesis by UV-radiation and methyl-methanesulphonate (MMS) was studied in mouse lymphocytes and leukemic cells by means of autoradiography and scintillation counting, after labelling in vitro with tritiated thymidine ([3H]dThd). Repair stimulation was detected by both procedures in LSTRA and YC8 leukemic cell lines as well as in primary fibroblasts of BALB/c and BALB/Mo mice. No stimulation was observed in primary cultures of lymphocytes from the spleen, thymus and lymph-nodes of the same mice. In primary lymphocytes neither stimulation with concanavalin A (Con A) nor pre-incubation with 5-bromodeoxyuridine (BUdR) were effective in making evident DNA repair. The data put into question the reliability of the repair test for the prediction of carcinogenic potential of chemicals.
在用氚标记的胸腺嘧啶核苷（[3H]dThd）进行体外标记后，通过放射自显影和闪烁计数法，研究了紫外线辐射和甲基磺酸甲酯（MMS）在小鼠淋巴细胞和白血病细胞中诱导DNA修复合成的情况。在LSTRA和YC8白血病细胞系以及BALB/c和BALB/Mo小鼠的原代成纤维细胞中，两种方法均检测到修复刺激。在相同小鼠的脾脏、胸腺和淋巴结的淋巴细胞原代培养物中未观察到刺激。在原代淋巴细胞中，用伴刀豆球蛋白A（Con A）刺激或用5-溴脱氧尿苷（BUdR）预孵育均不能有效地使DNA修复显现出来。这些数据对用于预测化学物质致癌潜力的修复试验的可靠性提出了质疑。
