Bull A W, Marnett L J, Dawe E J, Nigro N D
Carcinogenesis. 1983;4(2):207-10. doi: 10.1093/carcin/4.2.207.
The incorporation of tritiated deoxythymidine ([3H]dThd) into colonic DNA of male Sprague-Dawley rats treated intrarectally (i.r.) with bile salts and other substances has been investigated. Instillation of sodium deoxycholate caused an increase in the incorporation of [3H]dThd which was maximal 12 h after treatment. The level of incorporation showed a steep linear dose response from 0.5 mM to 15 mM bile salt. Higher concentrations of deoxycholate up to 300 mM only slightly increased the extent of incorporation when compared to the lower concentration. Several other substances also increased the extent of [3H]dThd incorporation; these include: chenodeoxycholate, lithocholate, sodium dodecyl sulfate, dioctyl sulfosuccinate, corn oil, beef fat, and trioctanoin. Substances which had no effect on [3H]dThd incorporation include cholesterol, dehydrocholate, sodium acetate, dextrose, and mineral oil. Many of the agents which increase colonic [3H]dThd incorporation are also known to enhance colonic tumorigenesis. These findings indicate similarities between the short-term effects, in their respective target tissues, of colon tumor promoters, and classical mouse skin tumor promoters.
已对经直肠（i.r.）用胆汁盐和其他物质处理的雄性Sprague-Dawley大鼠结肠DNA中氚标记脱氧胸苷（[3H]dThd）的掺入情况进行了研究。滴注脱氧胆酸钠导致[3H]dThd掺入增加，在处理后12小时达到最大值。掺入水平在0.5 mM至15 mM胆汁盐范围内呈现陡峭的线性剂量反应。与较低浓度相比，高达300 mM的较高浓度脱氧胆酸盐仅略微增加了掺入程度。其他几种物质也增加了[3H]dThd的掺入程度；这些物质包括：鹅脱氧胆酸盐、石胆酸盐、十二烷基硫酸钠、二辛基磺基琥珀酸酯、玉米油、牛肉脂肪和三辛酸甘油酯。对[3H]dThd掺入没有影响的物质包括胆固醇、脱氢胆酸盐、醋酸钠、葡萄糖和矿物油。许多增加结肠[3H]dThd掺入的试剂也已知会增强结肠肿瘤发生。这些发现表明结肠肿瘤启动子与经典小鼠皮肤肿瘤启动子在各自靶组织中的短期效应之间存在相似性。
