Warnick G R, Benderson J M, Albers J J
Clin Chem. 1983 Mar;29(3):516-9.
Proficiency surveys of Seattle-area laboratories suggest only slight improvement in overall performance in high-density lipoprotein (HDL) measurement between 1978 and 1982, although the reported workload for HDL has increased by 15%. The mean interlaboratory SD was 64 mg/L (ranging from 34 for a pool averaging 299 mg/L to 136 for a pool averaging 886 mg of HDL cholesterol per liter) in 1982, compared with 79 mg/L (range 48-155) in 1978-79. Of the individual laboratory results in the current survey, 39% deviated by more than 50 mg/L from target values as compared with 37% in 1978-79. The discrepant values were primarily ascribable to method inaccuracy: only 30% of laboratories in 1982 reported results that averaged within 30 mg/L of target values (vs 50% in 1978). For within-run precision, 80% of laboratories in 1982 had SDs of less than 30 mg/L, vs 70% in 1978. The 1982 survey included a lyophilized serum prepared by spray freezing and bulk lyophilization (Hyland Omega), identical to the pools used in the College of American Pathologists Comprehensive Chemistry Survey, and five pools of frozen plasma. Interlaboratory variation and biases for the Omega pool were similar to those for the frozen pools.
对西雅图地区实验室的能力验证调查表明，1978年至1982年间，高密度脂蛋白（HDL）测量的整体性能仅有轻微改善，尽管报告的HDL工作量增加了15%。1982年实验室间的平均标准差为64mg/L（范围从平均299mg/L的混合样本的34mg/L到平均每升含886mg HDL胆固醇的混合样本的136mg/L），而1978 - 1979年为79mg/L（范围48 - 155mg/L）。在当前调查的各个实验室结果中，39%的结果与目标值偏差超过50mg/L，而1978 - 1979年为37%。差异值主要归因于方法不准确：1982年只有30%的实验室报告的结果平均在目标值的30mg/L范围内（1978年为50%）。对于批内精密度，1982年80%的实验室标准差小于30mg/L，1978年为70%。1982年的调查包括一份通过喷雾冷冻和批量冻干制备的冻干血清（Hyland Omega），与美国病理学家学会综合化学能力验证中使用的混合样本相同，以及五份冷冻血浆混合样本。Omega混合样本的实验室间变异和偏差与冷冻混合样本相似。
