Warnick G R, Mayfield C, Albers J J
Clin Chem. 1981 Jan;27(1):116-23.
Commercial quality-control materials and frozen plasma and serum pools were evaluated for high-density lipoprotein (HDL) cholesterol quantitation by the heparin-Mn2+ and phosphotungstate-Mg2+ precipitation methods and by ultracentrifugal and electrophoretic methods. We evaluated among-day precision; inter-method differences in HDL cholesterol quantitation; lipid composition and electrophoretic mobility of th lipoprotein density fractions; and the effects of modifications in reagent concentration and temperature. Frozen plasma pools (in-house) and frozen serum pools (prepared by the Center for Disease Control) were determined to be suitable HDL controls. Also, a commercial lyophilized serum free of very-low-density lipoprotein (VLDL) to which VLDL is added in a diluent solution (Analytical Control) also appeared suitable as an HDL control material. Conventional lyophilized pools (Lyphochek Elevated, Monitrol I, Ortho Normal, Precilip) were less suitable as control materials because of poor between-day precision for one or both of the precipitation methods and, in some cases, because of large absolute differences observed between the HDL methods. A serum pool stabilized with ethylene glycol (Beckman Level I) was the least as appropriate as an HDL control material. Other lyophilized pools--Lipid Fraction Control (Hyland) prepared by a modified lyophilization process and Lipidophor (Immuno Diagnostika) prepared by lyophilization with a stabilizing agent--appear promising.
通过肝素 - Mn²⁺和磷钨酸 - Mg²⁺沉淀法、超速离心法及电泳法，对商业质量控制材料以及冷冻血浆和血清混合样本进行了高密度脂蛋白（HDL）胆固醇定量评估。我们评估了日间精密度；HDL胆固醇定量的方法间差异；脂蛋白密度组分的脂质组成和电泳迁移率；以及试剂浓度和温度变化的影响。冷冻血浆混合样本（内部制备）和冷冻血清混合样本（由疾病控制中心制备）被确定为合适的HDL对照。此外，一种不含极低密度脂蛋白（VLDL）的商业冻干血清，在稀释溶液中加入VLDL后（分析对照），似乎也适合作为HDL对照材料。传统的冻干混合样本（Lyphochek Elevated、Monitrol I、Ortho Normal、Precilip）不太适合作为对照材料，因为一种或两种沉淀方法的日间精密度较差，并且在某些情况下，由于HDL方法之间观察到的绝对差异较大。用乙二醇稳定的血清混合样本（贝克曼一级）最不适合作为HDL对照材料。其他冻干混合样本——通过改良冻干工艺制备的脂质组分对照（海兰）和通过添加稳定剂冻干制备的Lipidophor（免疫诊断）——似乎很有前景。
