Poole T W, Parke D V
FEBS Lett. 1983 Jan 10;151(1):27-30. doi: 10.1016/0014-5793(83)80335-4.
The radiometric assay of degranulation of the hepatic endoplasmic reticulum by chemical carcinogens has been re-examined. Both 1,2,3,4,- and 1,25,6-dibenzanthracenes caused degranulation of rough membranes in vitro; with acetamidofluorenes and naphthylamines the carcinogenic analogues caused moderately greater degranulation. Degranulation by 1,2,3,4-dibenzantracene was rapid and was maximal after 5 min incubation. Pretreatment of animals with phenobarbital or methylcholanthrene increased the fraction of rough membranes, but these were not fully granulated. The assay is limited in specificity and sensitivity because the 1.35 M sucrose gradient does not effectively separate rough and smooth membranes, and sedimented membranes are contaminated with aggregates of free ribosomes.
已对化学致癌物引起的肝内质网脱粒的放射性测定法进行了重新研究。1,2,3,4-二苯并蒽和1,2,5,6-二苯并蒽在体外均引起粗面内质网脱粒；对于乙酰氨基芴和萘胺，致癌类似物引起的脱粒程度稍大。1,2,3,4-二苯并蒽引起的脱粒迅速，孵育5分钟后达到最大值。用苯巴比妥或甲基胆蒽预处理动物可增加粗面内质网的比例，但这些粗面内质网并未完全颗粒化。该测定法在特异性和敏感性方面存在局限性，因为1.35M蔗糖梯度不能有效地分离粗面和滑面内质网，且沉淀的内质网被游离核糖体聚集体污染。
