Han M H, Seong B L, Son H J, Mheen T I
FEBS Lett. 1983 Jan 10;151(1):36-40. doi: 10.1016/0014-5793(83)80337-8.
It was found that enzyme from a microbial strain, Monocillium spp. ATCC 20621, catalyzed the oxidative reaction of rifamycin B to form rifamycin O. The identification of the reaction products suggested that the reaction proceeded by the oxidative cyclization of rifamycin B to give rifamycin O, which spontaneously hydrolyzed to rifamycin S in neutral aqueous milieu. The characteristic of the enzyme was different as compared with that of other polyphenol oxidases such as laccase. It is proposed that this new type of enzyme be classified into a subgroup EC 1.10.3.6 with a trivial name rifamycin B oxidase.
研究发现，来自微生物菌株单孢霉属（Monocillium spp.）ATCC 20621的酶催化利福霉素B的氧化反应生成利福霉素O。反应产物的鉴定表明，该反应是通过利福霉素B的氧化环化生成利福霉素O，利福霉素O在中性水介质中自发水解为利福霉素S。与漆酶等其他多酚氧化酶相比，该酶的特性有所不同。建议将这种新型酶归类为EC 1.10.3.6亚组，俗名利福霉素B氧化酶。
