Banerjee U C
Biochemical Engineering Research and Process Development Centre, Institute of Microbial Technology, Chandigarh, India.
Enzyme Microb Technol. 1993 Dec;15(12):1037-41. doi: 10.1016/0141-0229(93)90051-3.
Growing and resting cell systems of Curvularia lunata were used for the transformation of rifamycin B to rifamycin S. In the case of growing cells, rifamycin B was added at the time of inoculation and at the different phases of growth. Interestingly, it was found that C. lunata could grow in the presence of rifamycin B and could convert rifamycin B to rifamycin S. Growing cells 24 and 48 h of age were capable of transforming rifamycin B. Resting cells, cultivated at the exponential or early stationary phase, were found to be very active, and the resting cells of different ages were repeatedly used for the transformation reaction. Growing cells of 72 and 96 h were not capable of transforming rifamycin B, whereas resting cells of similar ages were very active. Due to the adsorption of rifamycins by the growing and resting cells of C. lunata, the stoichiometric yield of rifamycin S was not obtained.
新月弯孢霉的生长细胞体系和静止细胞体系被用于将利福霉素B转化为利福霉素S。对于生长细胞，在接种时以及生长的不同阶段添加利福霉素B。有趣的是，发现新月弯孢霉能够在利福霉素B存在的情况下生长，并能将利福霉素B转化为利福霉素S。24小时和48小时龄的生长细胞能够转化利福霉素B。在指数期或早期稳定期培养的静止细胞被发现非常活跃，不同年龄的静止细胞被反复用于转化反应。72小时和96小时的生长细胞不能转化利福霉素B，而相似年龄的静止细胞则非常活跃。由于新月弯孢霉的生长细胞和静止细胞对利福霉素的吸附作用，未获得利福霉素S的化学计量产率。
