[对-[双-(2-氯乙基)氨基]苯基]乙酸的同氮杂甾体酯（ASE）与艾氏腹水瘤细胞DNA的相互作用
Interaction of homo-aza-steroidal ester of [p-[bis-(2-chloroethyl) amino]phenyl]acetic acid (ASE) with DNA of Ehrlich ascites tumor cells.
作者信息
Papageorgiou A, Ivanov I G, Markov G G, Koliais S I, Boutis L, Catsoulakos P
出版信息
FEBS Lett. 1983 Mar 7;153(1):194-8. doi: 10.1016/0014-5793(83)80146-x.
A cytostatic, homo-aza-steroidal ester of [p-[bis-(2-chloroethyl) amino]phenyl]acetic acid (ASE) was reduced with NaB3H4 and [3H]ASE-treated DNA prepared in vitro. We found that: (1) ASE reacts preferentially with purines; (2) ASE decreases the thermal stability of the double helix upon binding to DNA; (3) [3H]ASE binding sites are clustered along the DNA molecules; (4) ASE binding sites probably represent oligo- or polypurine sequences.
用硼氢化钠还原[对-双（2-氯乙基）氨基]苯基]乙酸（ASE）的一种细胞生长抑制剂、同氮杂甾体酯，并制备了经[³H]ASE处理的体外DNA。我们发现：（1）ASE优先与嘌呤反应；（2）ASE与DNA结合后会降低双螺旋的热稳定性；（3）[³H]ASE结合位点沿DNA分子成簇分布；（4）ASE结合位点可能代表寡聚或多聚嘌呤序列。
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